BMRB Entry 16246

Name: Resonance Assignments for Yeast Prp24-RRM2
Published: 2009-09-04

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PDB ID: 2kh9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16246
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Resonance Assignments for Yeast Prp24-RRM2 PubMed: 19693704

Deposition date: 2009-04-07 Original release date: 2009-09-04

Authors: Martin-Tumasz, Stephen; Reiter, Nicholas; Butcher, Samuel

Citation: Martin-Tumasz, Stephen; Butcher, Samuel. "(1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA." Biomol. NMR Assignments 3, 227-230 (2009).

Assembly members:
Prp24-RRM2, polymer, 92 residues, 10012.586 Da.

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Prp24-RRM2: MTECTLWMTNFPPSYTQRNI RDLLQDINVVALSIRLPSLR FNTSRRFAYIDVTSKEDARY CVEKLNGLKIEGYTLVTKVS NPLELEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	257
15N chemical shifts	83
1H chemical shifts	522

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Prp24-RRM2	1

Entities:

Entity 1, Prp24-RRM2 92 residues - 10012.586 Da.

Final 8 residues are non-native due to 6x His tag

1

MET

THR

GLU

CYS

THR

LEU

TRP

MET

THR

ASN

2

PHE

PRO

SER

TYR

THR

GLN

ARG

ASN

ILE

3

ARG

ASP

LEU

GLN

ASP

ILE

ASN

VAL

4

ALA

LEU

SER

ILE

ARG

LEU

PRO

SER

LEU

ARG

5

PHE

ASN

THR

SER

ARG

PHE

ALA

TYR

ILE

6

ASP

VAL

THR

SER

LYS

GLU

ASP

ALA

ARG

TYR

7

CYS

VAL

GLU

LYS

LEU

ASN

GLY

LEU

LYS

ILE

8

GLU

GLY

TYR

THR

LEU

VAL

THR

LYS

VAL

SER

9

ASN

PRO

LEU

GLU

LEU

GLU

HIS

10

HIS

Samples:

Fully_Labeled: Prp24-RRM2, [U-99% 13C; U-99% 15N], 500 mM; TRIS 10 mM; potassium chloride 50 mM; DTT 1 mM; H2O 90%; D2O 10%

Referencing: Prp24-RRM2, [U-99% 13C; U-99% 15N], 500 uM; TRIS 10 mM; potassium chloride 50 mM; DTT 1 mM; DSS 10 uM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Fully_Labeled	isotropic	sample_conditions_1
3D CBCA(CO)NH	Fully_Labeled	isotropic	sample_conditions_1
3D C(CO)NH	Fully_Labeled	isotropic	sample_conditions_1
3D HNCO	Fully_Labeled	isotropic	sample_conditions_1
3D HNCACB	Fully_Labeled	isotropic	sample_conditions_1
3D HBHA(CO)NH	Fully_Labeled	isotropic	sample_conditions_1
3D HCCH-TOCSY	Fully_Labeled	isotropic	sample_conditions_1
3D H(CCO)NH	Fully_Labeled	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16230 16243 16244
PDB	2GHP 2GO9 2KH9 4N0T
DBJ	GAA25719
EMBL	CAA89251 CAY82100
GB	AAU09775 AHY76723 AJP40962 AJS62134 AJS62569
REF	NP_013995
SP	P49960
TPG	DAA10168

Biological Magnetic Resonance Data Bank