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Biological Magnetic Resonance Data Bank


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BMRB Entry 16396

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16396
MolProbity Validation Chart

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Title: The solution structure of CBD of human MCM6   PubMed: 20202939

Deposition date: 2009-07-08 Original release date: 2010-05-20

Authors: Liu, Changdong

Citation: Wei, Zhun; Liu, Changdong; Wu, Xing; Xu, Naining; Zhou, Bo; Liang, Chun; Zhu, Guang. "Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6."  J. Biol. Chem. 285, 12469-12473 (2010).

Assembly members:
CBD, polymer, 114 residues, 13304.011 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CBD: APKASLRLGFSEYSRISNLI VLHLRKVEEEEDESALKRSE LVNWYLKEIESEIDSEEELI NKKRIIEKVIHRLTHYDHVL IELTQAGLKGSTEGSESYEE DPYLVVNPNYLLED

Data sets:
Data typeCount
13C chemical shifts414
15N chemical shifts109
1H chemical shifts749

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBD1

Entities:

Entity 1, CBD 114 residues - 13304.011 Da.

1   ALAPROLYSALASERLEUARGLEUGLYPHE
2   SERGLUTYRSERARGILESERASNLEUILE
3   VALLEUHISLEUARGLYSVALGLUGLUGLU
4   GLUASPGLUSERALALEULYSARGSERGLU
5   LEUVALASNTRPTYRLEULYSGLUILEGLU
6   SERGLUILEASPSERGLUGLUGLULEUILE
7   ASNLYSLYSARGILEILEGLULYSVALILE
8   HISARGLEUTHRHISTYRASPHISVALLEU
9   ILEGLULEUTHRGLNALAGLYLEULYSGLY
10   SERTHRGLUGLYSERGLUSERTYRGLUGLU
11   ASPPROTYRLEUVALVALASNPROASNTYR
12   LEULEUGLUASP

Samples:

sample_1: CBD, [U-100% 13C; U-100% 15N], 0.5 mM; MES 50 mM; EDTA 1 mM; Glycerol 5%; NaCl 500 mM; H2O 90%; D2O 10%

sample_2: CBD, [U-100% 13C; U-100% 15N], 0.5 mM; MES 50 mM; EDTA 1 mM; Glycerol 5%; NaCl 500 mM; D2O 100%

sample_3: CBD 0.5 mM; MES 50 mM; EDTA 1 mM; Glycerol 5%; NaCl 500 mM; D2O 100%

sample_conditions_1: ionic strength: 0.5 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

CNS v1.1, A.T. Brunger - refinement

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 16796 17702
PDB
DBJ BAA12699 BAE01174 BAG35469 BAG61268
GB AAB48165 AAC50766 AAH32374 AAI12449 AAO26043
REF NP_001039699 NP_001247544 NP_001270984 NP_005906 XP_001154732
SP Q14566 Q2KIZ8

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts