BMRB Entry 16466
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16466
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Title: Solution structure and functional characterization of human plasminogen kringle 5 PubMed: 19821587
Deposition date: 2009-08-21 Original release date: 2009-12-11
Authors: Battistel, Marcos; Grishaev, Alexander; An, Seong Soo; Castellino, Francis; Llinas, Miguel
Citation: Battistel, Marcos; Grishaev, Alexander; An, Seong Soo A; Castellino, Francis; Llinas, Miguel. "Solution structure and functional characterization of human plasminogen kringle 5." Biochemistry 48, 10208-10219 (2009).
Assembly members:
rK5, polymer, 86 residues, 9569.646 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
rK5: SNADCMFGNGKGYRGKRVTT
VTGTPCQDWAAQEPHRHSIF
TPETNPRAGLEKNYCRNPDG
DVGGPWCYTTNPRKLYDYCD
VPQCAA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 95 |
| 1H chemical shifts | 469 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rK5 | 1 |
Entities:
Entity 1, rK5 86 residues - 9569.646 Da.
| 1 | SER | ASN | ALA | ASP | CYS | MET | PHE | GLY | ASN | GLY | ||||
| 2 | LYS | GLY | TYR | ARG | GLY | LYS | ARG | VAL | THR | THR | ||||
| 3 | VAL | THR | GLY | THR | PRO | CYS | GLN | ASP | TRP | ALA | ||||
| 4 | ALA | GLN | GLU | PRO | HIS | ARG | HIS | SER | ILE | PHE | ||||
| 5 | THR | PRO | GLU | THR | ASN | PRO | ARG | ALA | GLY | LEU | ||||
| 6 | GLU | LYS | ASN | TYR | CYS | ARG | ASN | PRO | ASP | GLY | ||||
| 7 | ASP | VAL | GLY | GLY | PRO | TRP | CYS | TYR | THR | THR | ||||
| 8 | ASN | PRO | ARG | LYS | LEU | TYR | ASP | TYR | CYS | ASP | ||||
| 9 | VAL | PRO | GLN | CYS | ALA | ALA |
Samples:
sample_1: D2O 10%; H2O 90%; sodium phosphate 50 mM; AMCHA 11 mM; rK5 2.2 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 5.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA, Linge, O, . - chemical shift assignment, refinement, structure solution
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
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