BMRB Entry 16484
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16484
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Title: NMR structure of CIP75 UBA domain PubMed: 20127391
Deposition date: 2009-09-08 Original release date: 2010-02-04
Authors: Kieken, Fabien; Spagnol, Gaelle; Su, Vivian; Lau, Alan; Sorgen, Paul
Citation: Kieken, Fabien; Spagnol, Gaelle; Su, Vivian; Lau, Alan; Sorgen, Paul. "NMR structure note: UBA domain of CIP75." J. Biomol. NMR 46, 245-250 (2010).
Assembly members:
CIP75_UBA, polymer, 53 residues, 5698.4 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CIP75_UBA: GPLGSMPEVRFQQQLEQLNS
MGFINREANLQALIATGGDI
NAAIERLLGSQLS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 162 |
| 15N chemical shifts | 58 |
| 1H chemical shifts | 379 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CIP75_UBA | 1 |
Entities:
Entity 1, CIP75_UBA 53 residues - 5698.4 Da.
Residues 1-5 represent a non-native cloning sequence.
| 1 | GLY | PRO | LEU | GLY | SER | MET | PRO | GLU | VAL | ARG | ||||
| 2 | PHE | GLN | GLN | GLN | LEU | GLU | GLN | LEU | ASN | SER | ||||
| 3 | MET | GLY | PHE | ILE | ASN | ARG | GLU | ALA | ASN | LEU | ||||
| 4 | GLN | ALA | LEU | ILE | ALA | THR | GLY | GLY | ASP | ILE | ||||
| 5 | ASN | ALA | ALA | ILE | GLU | ARG | LEU | LEU | GLY | SER | ||||
| 6 | GLN | LEU | SER |
Samples:
sample_1: CIP75 UBA, [U-13C; U-15N], 1.5-2.0 mM; D2O 7%; H2O 93%
sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature controller setting: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
ARIA, Linge, O, . - geometry optimization, refinement, structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB40326 BAC36837 BAD92803 BAG37034 BAG64068 |
| GB | AAF19084 AAF80171 AAH06410 AAH17686 AAH18403 |
| REF | NP_001291271 NP_064516 NP_277068 XP_001117405 XP_001163250 |
| SP | Q99NB8 Q9NRR5 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts