BMRB Entry 16550
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16550
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Title: Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO PubMed: 20153292
Deposition date: 2009-10-11 Original release date: 2010-02-18
Authors: Merkulova, Maria; Bakulina, Anastasia; Thaker, Youg; Gruber, Gerhard; Marshansky, Vladimir
Citation: Merkulova, Maria; Bakulina, Anastasia; Thaker, Youg Raj; Gruber, Gerhard; Marshansky, Vladimir. "Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO." Biochim. Biophys. Acta 1797, 1398-1409 (2010).
Assembly members:
ARNO(375-400), polymer, 26 residues, 3076 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
ARNO(375-400): VSVDPFYEMLAARKKRISVK
KKQEQP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 156 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PB domain | 1 |
Entities:
Entity 1, PB domain 26 residues - 3076 Da.
Residues from 375-400 represents PB (Polybasic)of ARNO protein.
| 1 | VAL | SER | VAL | ASP | PRO | PHE | TYR | GLU | MET | LEU | ||||
| 2 | ALA | ALA | ARG | LYS | LYS | ARG | ILE | SER | VAL | LYS | ||||
| 3 | LYS | LYS | GLN | GLU | GLN | PRO |
Samples:
sample_1: ARNO(375-400), [U-100% 15N], 1-2 mM
sample_conditions_1: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v2.6.9, Goddard - chemical shift assignment, chemical shift calculation, data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA33429 BAA33430 BAA33431 BAA33432 BAA87927 |
| EMBL | CAA68084 |
| GB | AAB09591 AAB41444 AAC77924 AAH04361 AAH04662 |
| REF | NP_001070562 NP_001106171 NP_001135984 NP_001137592 NP_001247820 |
| SP | P63034 P63035 Q2KI41 Q76MY7 Q99418 |
| TPG | DAA19658 |