BMRB Entry 16621
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16621
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Title: 1H, 13C, and 15N chemical shift assignment of KCNE3 in isotropic bicelles PubMed: 20044833
Deposition date: 2009-12-02 Original release date: 2010-02-11
Authors: Kang, Congbao; Vanoye, Carlos; Welch, Richard; Sanders, Charles
Citation: Kang, Congbao; Vanoye, Carlos; Welch, Richard; Van Horn, Wade; Sanders, Charles. "Functional delivery of a membrane protein into oocyte membranes using bicelles." Biochemistry 49, 653-655 (2010).
Assembly members:
KCNE3, polymer, 111 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
KCNE3: MGHHHHHHGMETTNGTETWY
ESLHAVLKALNATLHSNLLC
RPGPGLGPDNQTERRASLPG
RDDNSYMYILFVMFLFAVTV
GSLILGYTRSRKVDKRSDPY
HVYIKNRVSMI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 284 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 95 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KCNE3 | 1 |
Entities:
Entity 1, KCNE3 111 residues - Formula weight is not available
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | GLY | MET | ||||
| 2 | GLU | THR | THR | ASN | GLY | THR | GLU | THR | TRP | TYR | ||||
| 3 | GLU | SER | LEU | HIS | ALA | VAL | LEU | LYS | ALA | LEU | ||||
| 4 | ASN | ALA | THR | LEU | HIS | SER | ASN | LEU | LEU | CYS | ||||
| 5 | ARG | PRO | GLY | PRO | GLY | LEU | GLY | PRO | ASP | ASN | ||||
| 6 | GLN | THR | GLU | ARG | ARG | ALA | SER | LEU | PRO | GLY | ||||
| 7 | ARG | ASP | ASP | ASN | SER | TYR | MET | TYR | ILE | LEU | ||||
| 8 | PHE | VAL | MET | PHE | LEU | PHE | ALA | VAL | THR | VAL | ||||
| 9 | GLY | SER | LEU | ILE | LEU | GLY | TYR | THR | ARG | SER | ||||
| 10 | ARG | LYS | VAL | ASP | LYS | ARG | SER | ASP | PRO | TYR | ||||
| 11 | HIS | VAL | TYR | ILE | LYS | ASN | ARG | VAL | SER | MET | ||||
| 12 | ILE |
Samples:
KCNE3_in_bicelles: KCNE3, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; DTT 2 mM; EDTA 2 mM; bicelles 20%; D2O 10%; imidazole 250 mM; H2O 90%
sample_conditions_1: ionic strength: 0.250 M; pH: 6.5; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | KCNE3_in_bicelles | isotropic | sample_conditions_1 |
| 3D TROSY-HNCACB | KCNE3_in_bicelles | isotropic | sample_conditions_1 |
| 3D TROSY-CBCA(CO)NH | KCNE3_in_bicelles | isotropic | sample_conditions_1 |
| 3D TROSY-HNCA | KCNE3_in_bicelles | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | KCNE3_in_bicelles | isotropic | sample_conditions_1 |
| 3D HNCO | KCNE3_in_bicelles | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| GB | NM_005472 AAD28089 AAG16255 AAI10613 AAI13744 ABB00304 |
| BMRB | 16602 |
| DBJ | BAG34817 |
| EMBL | CAG33490 CAI56768 |
| REF | NP_005463 XP_001115704 XP_001174917 XP_002822287 XP_003777893 |
| SP | Q9Y6H6 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts