BMRB Entry 16633
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16633
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Title: Solution structure of Bv8 PubMed: 20677202
Deposition date: 2009-12-14 Original release date: 2010-08-12
Authors: Daly, Norelle; Craik, David
Citation: Morales, Rodrigo; Daly, Norelle; Vetter, Irina; Mobli, Mehdi; Napier, Ian; Craik, David; Lewis, Richard; Christie, Macdonald; King, Glenn; Alewood, Paul; Durek, Thomas. "Chemical synthesis and structure of the prokineticin bv8." Chembiochem 11, 1882-1888 (2010).
Assembly members:
prokineticin_Bv8, polymer, 77 residues, 8042.250 Da.
Natural source: Common Name: yellow-bellied toad Taxonomy ID: 8348 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bombina variegata
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
prokineticin_Bv8: AVITGACDKDVQCGSGTCCA
ASAWSRNIRFCIPLGNSGED
CHPASHKVPYDGKRLSSLCP
CKSGLTCSKSGEKFKCS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 443 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | prokineticin_Bv8 | 1 |
Entities:
Entity 1, prokineticin_Bv8 77 residues - 8042.250 Da.
| 1 | ALA | VAL | ILE | THR | GLY | ALA | CYS | ASP | LYS | ASP | ||||
| 2 | VAL | GLN | CYS | GLY | SER | GLY | THR | CYS | CYS | ALA | ||||
| 3 | ALA | SER | ALA | TRP | SER | ARG | ASN | ILE | ARG | PHE | ||||
| 4 | CYS | ILE | PRO | LEU | GLY | ASN | SER | GLY | GLU | ASP | ||||
| 5 | CYS | HIS | PRO | ALA | SER | HIS | LYS | VAL | PRO | TYR | ||||
| 6 | ASP | GLY | LYS | ARG | LEU | SER | SER | LEU | CYS | PRO | ||||
| 7 | CYS | LYS | SER | GLY | LEU | THR | CYS | SER | LYS | SER | ||||
| 8 | GLY | GLU | LYS | PHE | LYS | CYS | SER |
Samples:
sample_1: entity 1 mM
sample_conditions_1: ionic strength: 0 M; pH: 3.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
TOPSPIN, Bruker Biospin - collection
XEASY, Bartels et al. - data analysis
NMR spectrometers:
- Bruker Avance 900 MHz