BMRB Entry 16735
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16735
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Title: NMR resonance assignment of the apo C-terminal polypeptide of the Anthrax Lethal Factor catalytic domain PubMed: 20438702
Deposition date: 2010-02-18 Original release date: 2010-05-18
Authors: Dalkas, Georgios; Chasapis, Christos; Gkazonis, Petros; Bentrop, Detlef; Spyroulias, Georgios
Citation: Gkazonis, Petros; Dalkas, Georgios; Chasapis, Christos; Vlamis-Gardikas, Alexios; Bentrop, Detlef; Spyroulias, Georgios. "Purification and biophysical characterization of the core protease domain of anthrax lethal factor." Biochem. Biophys. Res. Commun. 396, 643-647 (2010).
Assembly members:
Anthrax_LF_C-terminal, polymer, 106 residues, 12122.4 Da.
Natural source: Common Name: Bacillus Anthracis Taxonomy ID: 1392 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus Anthracis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Anthrax_LF_C-terminal: GSKGVELRNDSEGFIHEFGH
AVDDYAGYLLDKNQSDLVTN
SKKFIDIFKEEGSNLTSYGR
TNEAEFFAEAFRLMHSTDHA
ERLKVQKNAPKTFQFINDQI
KFIINS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 264 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 88 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Anthrax Lethal Factor C-terminal | 1 |
Entities:
Entity 1, Anthrax Lethal Factor C-terminal 106 residues - 12122.4 Da.
| 1 | GLY | SER | LYS | GLY | VAL | GLU | LEU | ARG | ASN | ASP | ||||
| 2 | SER | GLU | GLY | PHE | ILE | HIS | GLU | PHE | GLY | HIS | ||||
| 3 | ALA | VAL | ASP | ASP | TYR | ALA | GLY | TYR | LEU | LEU | ||||
| 4 | ASP | LYS | ASN | GLN | SER | ASP | LEU | VAL | THR | ASN | ||||
| 5 | SER | LYS | LYS | PHE | ILE | ASP | ILE | PHE | LYS | GLU | ||||
| 6 | GLU | GLY | SER | ASN | LEU | THR | SER | TYR | GLY | ARG | ||||
| 7 | THR | ASN | GLU | ALA | GLU | PHE | PHE | ALA | GLU | ALA | ||||
| 8 | PHE | ARG | LEU | MET | HIS | SER | THR | ASP | HIS | ALA | ||||
| 9 | GLU | ARG | LEU | LYS | VAL | GLN | LYS | ASN | ALA | PRO | ||||
| 10 | LYS | THR | PHE | GLN | PHE | ILE | ASN | ASP | GLN | ILE | ||||
| 11 | LYS | PHE | ILE | ILE | ASN | SER |
Samples:
sample_1: Anthrax Lethal Factor, [U-98% 13C; U-98% 15N], 0.6 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAR79124 GAF01219 GAO62612 GAO68443 |
| EMBL | CAC93932 CAC93933 |
| GB | AAA22569 AAA79216 AAD32411 AAM26117 AAT28913 |
| REF | NP_052803 WP_001022096 WP_001022097 WP_001022099 WP_001022100 |
| SP | P15917 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts