BMRB Entry 16818
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16818
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Title: Solution structure of a novel Ubiquitin-binding domain from Human PLAA (PUBD, Gly76-Pro77 trans isomer) PubMed: 19423704
Deposition date: 2010-04-01 Original release date: 2010-05-11
Authors: Fu, Qing-Shan; Zhou, Chen-Jie; Gao, Hong-Chang; Lin, Dong-Hai; Hu, Hong-Yu
Citation: Fu, Qing-Shan; Zhou, Chen-Jie; Gao, Hong-Chang; Jiang, Ya-Jun; Zhou, Zi-Ren; Hong, Jing; Yao, Wen-Ming; Song, Ai-Xin; Lin, Dong-Hai; Hu, Hong-Yu. "Structural basis for ubiquitin recognition by a novel domain from human phospholipase A2-activating protein" J. Biol. Chem. 284, 19043-19052 (2009).
Assembly members:
PFUC_trans, polymer, 80 residues, 89.094 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PFUC_trans: ANQQTSGKVLYEGKEFDYVF
SIDVNEGGPSYKLPYNTSDD
PWLTAYNFLQKNDLNPMFLD
QVAKFIIDNTKGQMLGLGNP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 221 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 462 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PFUC_trans | 1 |
Entities:
Entity 1, PFUC_trans 80 residues - 89.094 Da.
| 1 | ALA | ASN | GLN | GLN | THR | SER | GLY | LYS | VAL | LEU | |
| 2 | TYR | GLU | GLY | LYS | GLU | PHE | ASP | TYR | VAL | PHE | |
| 3 | SER | ILE | ASP | VAL | ASN | GLU | GLY | GLY | PRO | SER | |
| 4 | TYR | LYS | LEU | PRO | TYR | ASN | THR | SER | ASP | ASP | |
| 5 | PRO | TRP | LEU | THR | ALA | TYR | ASN | PHE | LEU | GLN | |
| 6 | LYS | ASN | ASP | LEU | ASN | PRO | MET | PHE | LEU | ASP | |
| 7 | GLN | VAL | ALA | LYS | PHE | ILE | ILE | ASP | ASN | THR | |
| 8 | LYS | GLY | GLN | MET | LEU | GLY | LEU | GLY | ASN | PRO |
Samples:
sample_1: PUBD_trans, [U-100% 13C; U-100% 15N], 1 ± 0.02 mM; NaCl 50 mM; phosphate 20 mM; H2O 90%; D2O 100%
sample_2: PUBD_trans, [U-100% 13C; U-100% 15N], 1 ± 0.02 mM; NaCl 50 mM; phosphate 20 mM; D2O 100%
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Varian Unity 600 MHz
Related Database Links:
| BMRB | 16848 |
| PDB | |
| DBJ | BAA91803 BAA92105 BAD97264 BAE87781 BAG10899 |
| EMBL | CAB42881 CAB63739 |
| GB | AAD03030 AAD42075 AAH32551 ABW03596 ABW03904 |
| REF | NP_001026859 NP_001247477 NP_001270166 XP_002708092 XP_002743032 |
| SP | Q9Y263 |
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