BMRB Entry 16960
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16960
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of GxTX-1E PubMed: 20509680
Deposition date: 2010-05-27 Original release date: 2010-07-26
Authors: Lee, Seungkyu; Jung, Hyun Ho; Lee, Ju Yeon; Lee, Chul Won; Kim, Jae Il
Citation: Lee, Seungkyu; Milescu, Mirela; Jung, Hyun Ho; Lee, Ju Yeon; Bae, Chan Hyung; Lee, Chul Won; Kim, Ha Hyung; Swartz, Kenton; Kim, Jae Il. "Solution structure of GxTX-1E, a high-affinity tarantula toxin interacting with voltage sensors in Kv2.1 potassium channels ." Biochemistry 49, 5134-5142 (2010).
Assembly members:
GxTX-1E, polymer, 36 residues, 3948.66 Da.
Natural source: Common Name: spiders Taxonomy ID: 278060 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Plesiophrictus guangxiensis
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
GxTX-1E: EGECGGFWWKCGSGKPACCP
KYVCSPKWGLCNFPMP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 206 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GxTX-1E | 1 |
Entities:
Entity 1, GxTX-1E 36 residues - 3948.66 Da.
| 1 | GLU | GLY | GLU | CYS | GLY | GLY | PHE | TRP | TRP | LYS | ||||
| 2 | CYS | GLY | SER | GLY | LYS | PRO | ALA | CYS | CYS | PRO | ||||
| 3 | LYS | TYR | VAL | CYS | SER | PRO | LYS | TRP | GLY | LEU | ||||
| 4 | CYS | ASN | PHE | PRO | MET | PRO |
Samples:
sample_1: GxTX-1E 10 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 3.500; temperature: 310.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample_1 | solution | sample_conditions_1 |
| TOCSY | sample_1 | solution | sample_conditions_1 |
| NOESY | sample_1 | solution | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
CNS1.2 vany, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
SPARKY vany, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 0 MHz