BMRB Entry 16981
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16981
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Title: NMR Structure of the SARS Coronavirus Nonstructural Protein Nsp7 in Solution at pH 6.5 PubMed: 20709084
Deposition date: 2010-06-07 Original release date: 2010-07-08
Authors: Johnson, Margaret; Jaudzems, Kristaps; Wuthrich, Kurt
Citation: Johnson, Margaret; Jaudzems, Kristaps; Wuthrich, Kurt. "NMR Structure of the SARS-CoV Nonstructural Protein 7 in Solution at pH6.5." J. Mol. Biol. 402, 619-628 (2010).
Assembly members:
nsp7, polymer, 85 residues, 9472.080 Da.
Natural source: Common Name: SARS coronavirus Taxonomy ID: 227859 Superkingdom: virus Kingdom: not available Genus/species: Betacoronavirus Severe acute respiratory syndrome-related coronavirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
nsp7: GHSKMSDVKCTSVVLLSVLQ
QLRVESSSKLWAQCVQLHND
ILLAKDTTEAFEKMVSLLSV
LLSMQGAVDINRLCEEMLDN
RATLQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 630 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | nsp7 | 1 |
Entities:
Entity 1, nsp7 85 residues - 9472.080 Da.
| 1 | GLY | HIS | SER | LYS | MET | SER | ASP | VAL | LYS | CYS | ||||
| 2 | THR | SER | VAL | VAL | LEU | LEU | SER | VAL | LEU | GLN | ||||
| 3 | GLN | LEU | ARG | VAL | GLU | SER | SER | SER | LYS | LEU | ||||
| 4 | TRP | ALA | GLN | CYS | VAL | GLN | LEU | HIS | ASN | ASP | ||||
| 5 | ILE | LEU | LEU | ALA | LYS | ASP | THR | THR | GLU | ALA | ||||
| 6 | PHE | GLU | LYS | MET | VAL | SER | LEU | LEU | SER | VAL | ||||
| 7 | LEU | LEU | SER | MET | GLN | GLY | ALA | VAL | ASP | ILE | ||||
| 8 | ASN | ARG | LEU | CYS | GLU | GLU | MET | LEU | ASP | ASN | ||||
| 9 | ARG | ALA | THR | LEU | GLN |
Samples:
sample_1: nsp7, [U-98% 13C; U-98% 15N], 2 mM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 10 mM; sodium azide 3 mM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0.253 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-resolved [1H,1H]-NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C-resolved [1H,1H]-NOESY (aliphatic carbons) | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C-resolved [1H,1H]-NOESY (aromatic carbons) | sample_1 | isotropic | sample_conditions_1 |
| 2D [15N,1H]-HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HC(C)H-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D [13C,1H]-HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
UNIO v1.0.4, Torsten Herrmann - structure solution
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution
ATNOS v2.2, Torsten Herrmann, Peter G ntert, Kurt Wuthrich - structure solution
CANDID v2.2, Torsten Herrmann, Peter G ntert, Kurt Wuthrich - structure solution
OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement
TOPSPIN, Bruker Biospin - collection, data analysis
CARA, Keller and Wuthrich - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC81346 BAC81347 BAC81360 BAC81361 BAC81374 |
| GB | AAP13439 AAP13442 AAP13566 AAP13575 AAP30028 |
| REF | NP_828849 NP_828850 NP_828865 |
| SP | P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts