BMRB Entry 17014
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17014
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Title: Backbone 1H,13C and 15N assignments of ChxR PubMed: 24646934
Deposition date: 2010-06-22 Original release date: 2014-03-24
Authors: Hickey, John; Hefty, Scott
Citation: Barta, Michael; Hickey, John; Anbanandam, Asokan; Dyer, Kevin; Hammel, Michal; Hefty, P Scott. "Atypical response regulator ChxR from Chlamydia trachomatis is structurally poised for DNA binding." PLoS ONE 9, e91760-e91760 (2014).
Assembly members:
ChxR_effector_domain, polymer, 116 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ChxR_effector_domain: HSVPESIRFGPNVFYVLKLT
VETPEGSVHLTPSESGILKR
LLINKGQLCLRKHLLEEIKN
HAKAIVARNVDVHIASLRKK
LGAYGSRIVTLRGVGYLFSD
DGDKKFSQQDTKLSLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 452 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 728 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ChxR monomer | 1 |
Entities:
Entity 1, ChxR monomer 116 residues - Formula weight is not available
| 1 | HIS | SER | VAL | PRO | GLU | SER | ILE | ARG | PHE | GLY | ||||
| 2 | PRO | ASN | VAL | PHE | TYR | VAL | LEU | LYS | LEU | THR | ||||
| 3 | VAL | GLU | THR | PRO | GLU | GLY | SER | VAL | HIS | LEU | ||||
| 4 | THR | PRO | SER | GLU | SER | GLY | ILE | LEU | LYS | ARG | ||||
| 5 | LEU | LEU | ILE | ASN | LYS | GLY | GLN | LEU | CYS | LEU | ||||
| 6 | ARG | LYS | HIS | LEU | LEU | GLU | GLU | ILE | LYS | ASN | ||||
| 7 | HIS | ALA | LYS | ALA | ILE | VAL | ALA | ARG | ASN | VAL | ||||
| 8 | ASP | VAL | HIS | ILE | ALA | SER | LEU | ARG | LYS | LYS | ||||
| 9 | LEU | GLY | ALA | TYR | GLY | SER | ARG | ILE | VAL | THR | ||||
| 10 | LEU | ARG | GLY | VAL | GLY | TYR | LEU | PHE | SER | ASP | ||||
| 11 | ASP | GLY | ASP | LYS | LYS | PHE | SER | GLN | GLN | ASP | ||||
| 12 | THR | LYS | LEU | SER | LEU | GLU |
Samples:
sample_1: ChxR effector domain, [U-100% 13C; U-100% 15N], 1.0 mM; NaCl 400 mM; Na2HPO4 20 mM; KH2PO4 20 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAP04331 CAP07285 CAX09301 CAX10195 CAX11088 |
| GB | AAC68234 AAX50907 ADH17418 ADH18341 ADH19265 |
| REF | NP_220147 WP_009871999 WP_009872804 WP_009873961 WP_010725282 |
| SP | A0A0H3MDW1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts