BMRB Entry 17033
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17033
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Title: Solution NMR Structure of Nonsense mRNA reducing factor 3A from H. Sapiens, Northeast Structural Genomics Consortium Target HR4714B
Deposition date: 2010-06-30 Original release date: 2010-07-09
Authors: Mani, Rajeswari; Mao, Lei; Ciccosanti, Colleen; Shastry, Ritu; Acton, Thomas; Xiao, Rong; Swapna, G.V.T; Everett, John; Montelione, Gaetano
Citation: Mani, Rajeswari; Mao, Lei; Ciccosanti, Colleen; Shastry, Ritu; Acton, Thomas; Xiao, Rong; Swapna, G.V.T; Everett, John; Montelione, Gaetano. "olution NMR Structure of Nonsense mRNA reducing factor 3A from H. Sapiens, Northeast Structural Genomics Consortium Target HR4714B" Not known ., .-..
Assembly members:
entity, polymer, 97 residues, 11596.293 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MGHHHHHHSHMVVIRRLPPG
LTKEQLEEQLRPLPAHDYFE
FFAADLSLYPHLYSRAYINF
RNPDDILLFRDRFDGYIFLD
SKGLEYPAVVEFAPFQK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 288 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 552 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nonsense mRNA reducing factor | 1 |
Entities:
Entity 1, Nonsense mRNA reducing factor 97 residues - 11596.293 Da.
First 10 residue, MGHHHHHHSH is a N-terminal tag.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | SER | HIS | ||||
| 2 | MET | VAL | VAL | ILE | ARG | ARG | LEU | PRO | PRO | GLY | ||||
| 3 | LEU | THR | LYS | GLU | GLN | LEU | GLU | GLU | GLN | LEU | ||||
| 4 | ARG | PRO | LEU | PRO | ALA | HIS | ASP | TYR | PHE | GLU | ||||
| 5 | PHE | PHE | ALA | ALA | ASP | LEU | SER | LEU | TYR | PRO | ||||
| 6 | HIS | LEU | TYR | SER | ARG | ALA | TYR | ILE | ASN | PHE | ||||
| 7 | ARG | ASN | PRO | ASP | ASP | ILE | LEU | LEU | PHE | ARG | ||||
| 8 | ASP | ARG | PHE | ASP | GLY | TYR | ILE | PHE | LEU | ASP | ||||
| 9 | SER | LYS | GLY | LEU | GLU | TYR | PRO | ALA | VAL | VAL | ||||
| 10 | GLU | PHE | ALA | PRO | PHE | GLN | LYS |
Samples:
sample_1: HR4714B, [U-100% 13C; U-100% 15N], 0.82 mM; H2O 90%; D2O 10%; NaCl 100 mM; DTT 5 mM; NaN3 0.02%; Tris-HCl 10 mM
sample_2: HR4714B, [U-10% 13C; U-99% 15N], 0.9 mM; H2O 90%; D2O 10%; NaCl 100 mM; DTT 5 mM; NaN3 0.02%; Tris-HCl 10 mM
sample_conditions_1: ionic strength: 110 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_2 | isotropic | sample_conditions_1 |
| 3D simul NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D arom NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS v2.0.6, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution
AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - structure solution
PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE02295 BAG57751 |
| GB | AAG48510 AAG60690 EAX09248 EAX09249 EAX09250 |
| REF | NP_001272234 NP_075387 XP_002742608 XP_002824527 XP_003281173 |
| SP | Q9H1J1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts