BMRB Entry 17040
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17040
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Title: STRUCTURAL AND FUNCTIONAL ANALYSIS OF TM VI OF THE NHE1 ISOFORM OF THE Na+/H+ EXCHANGER PubMed: 20843797
Deposition date: 2010-07-01 Original release date: 2010-09-03
Authors: Tzeng, Jennifer; Lee, Brian; Sykes, Brian; Fliegel, Larry
Citation: Tzeng, Jennifer; Lee, Brian; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of Transmembrane Segment VI of the NHE1 Isoform of the Na+/H+ Exchanger." J. Biol. Chem. 285, 36656-36665 (2010).
Assembly members:
TM_VI, polymer, 32 residues, 3479.16 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
TM_VI: XKKDNLLFGSIISAVDPVAV
LAVFEEIHKKKX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 216 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TM VI | 1 |
Entities:
Entity 1, TM VI 32 residues - 3479.16 Da.
N-terminal acetyl, C-terminal NH2 capped polypeptide lysine tagged termini
| 1 | ALY | LYS | LYS | ASP | ASN | LEU | LEU | PHE | GLY | SER | ||||
| 2 | ILE | ILE | SER | ALA | VAL | ASP | PRO | VAL | ALA | VAL | ||||
| 3 | LEU | ALA | VAL | PHE | GLU | GLU | ILE | HIS | LYS | LYS | ||||
| 4 | LYS | NH2 |
Samples:
sample_1: TM VI 2 mM; DSS 0.25 mM; DPC, [U-2H], 150 mM
sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak assignment, peak picking
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
| BMRB | 19484 |
| PDB | |
| DBJ | BAE02258 BAE75800 |
| EMBL | CAA42558 CAA69925 CAF94405 CAG03374 CDQ60725 |
| GB | AAA31092 AAB20633 AAB59460 AAC60606 AAF21350 |
| REF | NP_001007104 NP_001038108 NP_001081553 NP_001095191 NP_001273957 |
| SP | P19634 P23791 P48762 |