BMRB Entry 17143
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17143
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the first dsRBD of protein HYL1 PubMed: 20735118
Deposition date: 2010-08-23 Original release date: 2010-09-24
Authors: Rasia, Rodolfo; Mateos, Julieta; Bologna, Nicolas; Burdisso, Paula; Imbert, Lionel; Palatnik, Javier; Boisbouvier, Jerome
Citation: Rasia, Rodolfo; Mateos, Julieta; Bologna, Nicolas; Burdisso, Paula; Imbert, Lionel; Palatnik, Javier; Boisbouvier, Jerome. "Structure and RNA Interactions of the Plant MicroRNA Processing-Associated Protein HYL1." Biochemistry 49, 8237-8239 (2010).
Assembly members:
HYL1_dsRBD_1, polymer, 103 residues, 8031.225 Da.
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HYL1_dsRBD_1: GHMMTSTDVSSGVSNCYVFK
SRLQEYAQKYKLPTPVYEIV
KEGPSHKSLFQSTVILDGVR
YNSLPGFFNRKAAEQSAAEV
ALRELAKSSELSQCVSQPVH
ETG
- assigned_chemical_shifts
- RDCs
| Data type | Count |
| 13C chemical shifts | 264 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 156 |
| residual dipolar couplings | 292 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HYL1_dsRBD_1-1 | 1 |
Entities:
Entity 1, HYL1_dsRBD_1-1 103 residues - 8031.225 Da.
| 1 | GLY | HIS | MET | MET | THR | SER | THR | ASP | VAL | SER | ||||
| 2 | SER | GLY | VAL | SER | ASN | CYS | TYR | VAL | PHE | LYS | ||||
| 3 | SER | ARG | LEU | GLN | GLU | TYR | ALA | GLN | LYS | TYR | ||||
| 4 | LYS | LEU | PRO | THR | PRO | VAL | TYR | GLU | ILE | VAL | ||||
| 5 | LYS | GLU | GLY | PRO | SER | HIS | LYS | SER | LEU | PHE | ||||
| 6 | GLN | SER | THR | VAL | ILE | LEU | ASP | GLY | VAL | ARG | ||||
| 7 | TYR | ASN | SER | LEU | PRO | GLY | PHE | PHE | ASN | ARG | ||||
| 8 | LYS | ALA | ALA | GLU | GLN | SER | ALA | ALA | GLU | VAL | ||||
| 9 | ALA | LEU | ARG | GLU | LEU | ALA | LYS | SER | SER | GLU | ||||
| 10 | LEU | SER | GLN | CYS | VAL | SER | GLN | PRO | VAL | HIS | ||||
| 11 | GLU | THR | GLY |
Samples:
Isotropic_sample: HYL1 dsRBD 1, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 100 mM; sodium chloride 50 mM; DTT 10 mM; sodium azide 0.05%; H2O 90%; D2O 10%
Aligned_sample: HYL1 dsRBD 1, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 100 mM; sodium chloride 50 mM; DTT 10 mM; sodium azide 0.05%; C12E5 5%; Hexanol 0.2%; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HNCO | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HNCA | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HNCACB | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HNCO JNH | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HNCOCA JCAHA | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HNCO JCACO | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HNCO JCH | Isotropic_sample | isotropic | sample_conditions_1 |
| 3D HNCO JNH | Aligned_sample | anisotropic | sample_conditions_1 |
| 3D HNCOCA JCAHA | Aligned_sample | anisotropic | sample_conditions_1 |
| 3D HNCO JCACO | Aligned_sample | anisotropic | sample_conditions_1 |
| 3D HNCO JCH | Aligned_sample | anisotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Varian DirectDrive 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts