BMRB Entry 17252
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17252
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Title: Solution NMR Structure of BCMA PubMed: 23438059
Deposition date: 2010-10-12 Original release date: 2013-06-04
Authors: Pellegrini, M.; Willen, L.; Perroud, M.; Krushinskie, D.; Strauch, K.; Cuervo, H.; Sun, Y.; Day, E.; Schneider, P.; Zheng, T.
Citation: Pellegrini, Maria; Willen, Laure; Perroud, Mai; Krushinskie, Dennis; Strauch, Kathy; Cuervo, Hernan; Day, Eric; Schneider, Pascal; Zheng, Timothy. "Structure of the extracellular domains of human and Xenopus Fn14: implications in the evolution of TWEAK and Fn14 interactions." FEBS J. 280, 1818-1829 (2013).
Assembly members:
BCMA, polymer, 50 residues, 5532.333 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis Host organism: N/A
Entity Sequences (FASTA):
BCMA: LQMAGQCSQNEYFDSLLHAC
IPCQLRCSSNTPPLTCQRYC
NASVTNSVKX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 182 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BCMA | 1 |
Entities:
Entity 1, BCMA 50 residues - 5532.333 Da.
an additional Cys (acetamidomethyl protected) was added to the C-terminus of the sythetic construct
| 1 | LEU | GLN | MET | ALA | GLY | GLN | CYS | SER | GLN | ASN | |
| 2 | GLU | TYR | PHE | ASP | SER | LEU | LEU | HIS | ALA | CYS | |
| 3 | ILE | PRO | CYS | GLN | LEU | ARG | CYS | SER | SER | ASN | |
| 4 | THR | PRO | PRO | LEU | THR | CYS | GLN | ARG | TYR | CYS | |
| 5 | ASN | ALA | SER | VAL | THR | ASN | SER | VAL | LYS | CY1 |
Samples:
sample: BCMA 900 uM; sodium phosphate 10 mM; sodium azide 0.02%; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample | isotropic | sample_conditions_1 |
Software:
CNS v1.1, Brunger A. T. et.al. - structure solution
SPARKY v3.11, Goddard - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz MHz
Related Database Links:
| PDB | |
| DBJ | BAB60895 BAI47121 |
| EMBL | CAA78679 CAA78680 CAA82690 CAA82691 |
| GB | AAB67251 AAH58291 AAR84240 AAV92616 AAX36273 |
| REF | NP_001183 XP_002826185 XP_003269345 XP_003832741 XP_004057277 |
| SP | Q02223 |