BMRB

Biological Magnetic Resonance Data Bank


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BMRB Entry 17288

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17288
MolProbity Validation Chart

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Title: 1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal toxin MazF   PubMed: 21213075

Deposition date: 2010-11-09 Original release date: 2011-01-10

Authors: Zorzini, Valentina; Cheung, Ambrose; Loris, Remy; van Nuland, Nico

Citation: Zorzini, Valentina; Haesaerts, Sarah; Cheung, Ambrose; Loris, Remy; van Nuland, Nico. "1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal MazF mRNA interferase."  Biomol. NMR Assignments 5, 157-160 (2011).

Assembly members:
SaMazF, polymer, 132 residues, Formula weight is not available

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SaMazF: GSSHHHHHHSQDPIRRGDVY LADLSPVQGSEQGGVRPVVI IQNDTGNKYSPTVIVAAITG RINKAKIPTHVEIEKKKYKL DKDSVILLEQIRTLDKKRLK EKLTYLSDDKMKEVDNALMI SLGLNAVAHQKN

Data sets:
Data typeCount
13C chemical shifts523
15N chemical shifts121
1H chemical shifts876

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SaMazF1

Entities:

Entity 1, SaMazF 132 residues - Formula weight is not available

1   GLYSERSERHISHISHISHISHISHISSER
2   GLNASPPROILEARGARGGLYASPVALTYR
3   LEUALAASPLEUSERPROVALGLNGLYSER
4   GLUGLNGLYGLYVALARGPROVALVALILE
5   ILEGLNASNASPTHRGLYASNLYSTYRSER
6   PROTHRVALILEVALALAALAILETHRGLY
7   ARGILEASNLYSALALYSILEPROTHRHIS
8   VALGLUILEGLULYSLYSLYSTYRLYSLEU
9   ASPLYSASPSERVALILELEULEUGLUGLN
10   ILEARGTHRLEUASPLYSLYSARGLEULYS
11   GLULYSLEUTHRTYRLEUSERASPASPLYS
12   METLYSGLUVALASPASNALALEUMETILE
13   SERLEUGLYLEUASNALAVALALAHISGLN
14   LYSASN

Samples:

sample_1: SaMazF, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian Uniform NMR System 800 MHz

Related Database Links:

PDB
DBJ BAB43155 BAB58230 BAB95857 BAF68246 BAF78936
EMBL CAA68928 CAA71064 CAG41137 CAG43780 CAI81642
GB AAW37020 ABD22408 ABD31337 ABQ49887 ABR52972
REF WP_000621174 WP_000621175 WP_000621176 WP_000621177 WP_000621178
SP A6QIR4 A7X4P5 Q2FF56 Q2FWI8 Q2YUI5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts