BMRB Entry 17303
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17303
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Title: 1H and 15N RESONANCE ASSIGNMENT OF HUMAN HOLO L-FABP
Deposition date: 2010-11-17 Original release date: 2012-06-05
Authors: Cai, Jun; Luecke, Christian; Chen, Zhongjing; Qiao, Ye; Klimtchuk, Elena; Hamilton, James
Citation: Cai, Jun; Luecke, Christian; Chen, Zhongjing; Qiao, Ye; Klimtchuk, Elena; Hamilton, James. "Solution Structure and Backbone Dynamics of Human Liver Fatty Acid Binding Protein: Fatty Acid Binding Revisited" Biophys. J. 102, 2585-2594 (2012).
Assembly members:
L-FABP, polymer, 126 residues, 14095.292 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
L-FABP: SFSGKYQLQSQENFEAFMKA
IGLPEELIQKGKDIKGVSEI
VQNGKHFKFTITAGSKVIQN
EFTVGEECELETMTGEKVKT
VVQLEGDNKLVTTFKNIKSV
TELNGDIITNTMTLGDIVFK
RISKRI
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 140 |
| 1H chemical shifts | 942 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human liver fatty acid binding protein | 1 |
Entities:
Entity 1, human liver fatty acid binding protein 126 residues - 14095.292 Da.
the N-terminal residue Met1 is missing; the assignments thus start with Ser2
| 1 | SER | PHE | SER | GLY | LYS | TYR | GLN | LEU | GLN | SER | ||||
| 2 | GLN | GLU | ASN | PHE | GLU | ALA | PHE | MET | LYS | ALA | ||||
| 3 | ILE | GLY | LEU | PRO | GLU | GLU | LEU | ILE | GLN | LYS | ||||
| 4 | GLY | LYS | ASP | ILE | LYS | GLY | VAL | SER | GLU | ILE | ||||
| 5 | VAL | GLN | ASN | GLY | LYS | HIS | PHE | LYS | PHE | THR | ||||
| 6 | ILE | THR | ALA | GLY | SER | LYS | VAL | ILE | GLN | ASN | ||||
| 7 | GLU | PHE | THR | VAL | GLY | GLU | GLU | CYS | GLU | LEU | ||||
| 8 | GLU | THR | MET | THR | GLY | GLU | LYS | VAL | LYS | THR | ||||
| 9 | VAL | VAL | GLN | LEU | GLU | GLY | ASP | ASN | LYS | LEU | ||||
| 10 | VAL | THR | THR | PHE | LYS | ASN | ILE | LYS | SER | VAL | ||||
| 11 | THR | GLU | LEU | ASN | GLY | ASP | ILE | ILE | THR | ASN | ||||
| 12 | THR | MET | THR | LEU | GLY | ASP | ILE | VAL | PHE | LYS | ||||
| 13 | ARG | ILE | SER | LYS | ARG | ILE |
Samples:
sample_1: potassium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.05%; human liver fatty acid binding protein 3 mM; potassium oleate, [U-13C], 15 mM; H2O 95%; D2O 5%
sample_2: potassium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.05%; human liver fatty acid binding protein, [U-15N], 2 mM; potassium oleate 10 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
DISCOVER v2000, Accelrys Software Inc. - refinement
NMRView v8.0, Johnson, One Moon Scientific - data analysis, peak picking
xwinnmr v3.5, Bruker Biospin - collection, processing
TOPSPIN v1.3, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 17302 19160 19188 19189 25333 |
| PDB | |
| DBJ | BAI46102 |
| EMBL | CAG46887 CAH90116 |
| GB | AAA52418 AAA52419 AAH22287 AAH32801 AAX37108 |
| REF | NP_001125017 NP_001434 XP_001140263 XP_003268834 XP_003805902 |
| SP | P07148 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts