BMRB Entry 17356

Name: Biophysical studies of lipid interacting regions of DGD2 in Arabidopsis thaliana
Published: 2011-05-05

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PDB ID: 2l7c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17356
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Biophysical studies of lipid interacting regions of DGD2 in Arabidopsis thaliana PubMed: 21506606

Deposition date: 2010-12-10 Original release date: 2011-05-05

Authors: Szpryngiel, Scarlett; Ge, Changrong; Iakovleva, Irina; Lind, Jesper; Wieslander, Ake; Maler, Lena

Citation: Szpryngiel, Scarlett; Ge, Changrong; Iakovleva, Irina; Georgiev, Alexander; Lind, Jesper; Wieslander, Ake; Maler, Lena. "Lipid Interacting Regions in Phosphate Stress Glycosyltransferase atDGD2 from Arabidopsis thaliana." Biochemistry 50, 4451-4466 (2011).

Assembly members:
atDGD2_S227_245, polymer, 19 residues, 2227.622 Da.

Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
atDGD2_S227_245: QPFTKGAYYIGKMVWSKGY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DGD2	1

Entities:

Entity 1, DGD2 19 residues - 2227.622 Da.

1

GLN

PRO

PHE

THR

LYS

GLY

ALA

TYR

ILE

2

GLY

LYS

MET

VAL

TRP

SER

LYS

GLY

TYR

Samples:

sample_1: atDGD2_S227_245 0.5 mM; DPC, [U-2H], 50 mM; D2O, [U-2H], 10%; sodium phosphate 50 mM; H2O 90%

sample_conditions_1: ionic strength: 50 mM; pH: 5.7; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 500 MHz