BMRB Entry 17377
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17377
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Title: Solution structure of the MFS-bound Sans CEN2 peptide PubMed: 21311020
Deposition date: 2010-12-22 Original release date: 2011-02-14
Authors: Pan, Lifeng; Wu, Lin; Wei, Zhiyi; Zhang, Mingjie
Citation: Wu, Lin; Pan, Lifeng; Wei, Zhiyi; Zhang, Mingjie. "Structure of MyTH4-FERM Domains in Myosin VIIa Tail Bound to Cargo." Science 331, 757-760 (2011).
Assembly members:
entity, polymer, 11 residues, 1315.396 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: EELPWDELDLG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 72 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sans CEN2 | 1 |
Entities:
Entity 1, Sans CEN2 11 residues - 1315.396 Da.
| 1 | GLU | GLU | LEU | PRO | TRP | ASP | GLU | LEU | ASP | LEU | ||||
| 2 | GLY |
Samples:
sample_1: sodium chloride 50 mM; DTT 1 mM; EDTA 1 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
PIPP, Garrett - chemical shift assignment, peak picking
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 750 MHz