BMRB Entry 17382
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17382
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Title: Solution structure of the human Raf-1 kinase inhibitor protein PubMed: 20924725
Deposition date: 2011-01-27 Original release date: 2011-02-15
Authors: Yi, C.
Citation: Yi, Cuiying; Peng, Yu; Guo, Chenyun; Lin, Donghai. "1H, 13C, 15N backbone and side-chain resonance assignments of the human Raf-1 kinase inhibitor protein." Biomol. NMR Assignments 5, 63-66 (2011).
Assembly members:
Raf-1_kinase_inhibitor_protein, polymer, 187 residues, 21086.967 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Raf-1_kinase_inhibitor_protein: MPVDLSKWSGPLSLQEVDEQ
PQHPLHVTYAGAAVDELGKV
LTPTQVKNRPTSISWDGLDS
GKLYTLVLTDPDAPSRKDPK
YREWHHFLVVNMKGNDISSG
TVLSDYVGSGPPKGTGLHRY
VWLVYEQDRPLKCDEPILSN
RSGDHRGKFKVASFRKKYEL
RAPVAGTCYQAEWDDYVPKL
YEQLSGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 755 |
| 15N chemical shifts | 181 |
| 1H chemical shifts | 1241 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Raf-1 kinase inhibitor protein | 1 |
Entities:
Entity 1, Raf-1 kinase inhibitor protein 187 residues - 21086.967 Da.
| 1 | MET | PRO | VAL | ASP | LEU | SER | LYS | TRP | SER | GLY | ||||
| 2 | PRO | LEU | SER | LEU | GLN | GLU | VAL | ASP | GLU | GLN | ||||
| 3 | PRO | GLN | HIS | PRO | LEU | HIS | VAL | THR | TYR | ALA | ||||
| 4 | GLY | ALA | ALA | VAL | ASP | GLU | LEU | GLY | LYS | VAL | ||||
| 5 | LEU | THR | PRO | THR | GLN | VAL | LYS | ASN | ARG | PRO | ||||
| 6 | THR | SER | ILE | SER | TRP | ASP | GLY | LEU | ASP | SER | ||||
| 7 | GLY | LYS | LEU | TYR | THR | LEU | VAL | LEU | THR | ASP | ||||
| 8 | PRO | ASP | ALA | PRO | SER | ARG | LYS | ASP | PRO | LYS | ||||
| 9 | TYR | ARG | GLU | TRP | HIS | HIS | PHE | LEU | VAL | VAL | ||||
| 10 | ASN | MET | LYS | GLY | ASN | ASP | ILE | SER | SER | GLY | ||||
| 11 | THR | VAL | LEU | SER | ASP | TYR | VAL | GLY | SER | GLY | ||||
| 12 | PRO | PRO | LYS | GLY | THR | GLY | LEU | HIS | ARG | TYR | ||||
| 13 | VAL | TRP | LEU | VAL | TYR | GLU | GLN | ASP | ARG | PRO | ||||
| 14 | LEU | LYS | CYS | ASP | GLU | PRO | ILE | LEU | SER | ASN | ||||
| 15 | ARG | SER | GLY | ASP | HIS | ARG | GLY | LYS | PHE | LYS | ||||
| 16 | VAL | ALA | SER | PHE | ARG | LYS | LYS | TYR | GLU | LEU | ||||
| 17 | ARG | ALA | PRO | VAL | ALA | GLY | THR | CYS | TYR | GLN | ||||
| 18 | ALA | GLU | TRP | ASP | ASP | TYR | VAL | PRO | LYS | LEU | ||||
| 19 | TYR | GLU | GLN | LEU | SER | GLY | LYS |
Samples:
sample_1: Raf-1 kinase inhibitor protein, [U-100% 13C; U-100% 15N], 3 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 200 mM; pH: 4.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 16992 18204 |
| PDB | |
| DBJ | BAA03684 BAE88027 BAE88359 BAG34868 BAG61396 |
| EMBL | CAA51652 CAA53031 CAA59404 CAH93256 |
| GB | AAB32876 AAD14234 AAH08714 AAH17396 AAH31102 |
| PRF | 2117380B |
| REF | NP_001126915 NP_001233128 NP_002558 XP_002753109 XP_004054017 |
| SP | P30086 P48737 Q5R4R0 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts