BMRB Entry 17420
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17420
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Title: Solution structure of Chr148 from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target Chr148
Deposition date: 2011-01-21 Original release date: 2011-02-04
Authors: Liu, Yizhou; Lee, Dan; Ciccosanti, Colleen; Nair, Lei MaoR; Rost, B.; Acton, T.B.; Xiao, R.; Everett, J.K; Montelione, G.T; Prestegard, James
Citation: Liu, Yizhou; Lee, Dan; Ciccosanti, Colleen; Nair, Lei MaoR; Rost, B; Acton, T.B.; Xiao, R.; Everett, J.K; Montelione, G.T; Prestegard, James. "Solution structure of Chr148 from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target Chr148" Not known ., .-..
Assembly members:
Chr148, polymer, 144 residues, 16413.268 Da.
Natural source: Common Name: CFb group bacteria Taxonomy ID: 985 Superkingdom: Bacteria Kingdom: not available Genus/species: Cytophaga hutchinsonii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Chr148: GNKITVEVTVYAAIEKVWKY
WNEPAHIMKWCQASPEWHVP
AAQNDLKAGGTFTTTMAAKD
GSMSFDFGGVYDQVKTNDLI
EYTIGDGRKVRIVFTHTGDT
TNIVESFDPEETNPRELQQS
GWQAILNSFKSYTENNLEHH
HHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 548 |
| 15N chemical shifts | 140 |
| 1H chemical shifts | 768 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Chr148 | 1 |
Entities:
Entity 1, Chr148 144 residues - 16413.268 Da.
| 1 | GLY | ASN | LYS | ILE | THR | VAL | GLU | VAL | THR | VAL | ||||
| 2 | TYR | ALA | ALA | ILE | GLU | LYS | VAL | TRP | LYS | TYR | ||||
| 3 | TRP | ASN | GLU | PRO | ALA | HIS | ILE | MET | LYS | TRP | ||||
| 4 | CYS | GLN | ALA | SER | PRO | GLU | TRP | HIS | VAL | PRO | ||||
| 5 | ALA | ALA | GLN | ASN | ASP | LEU | LYS | ALA | GLY | GLY | ||||
| 6 | THR | PHE | THR | THR | THR | MET | ALA | ALA | LYS | ASP | ||||
| 7 | GLY | SER | MET | SER | PHE | ASP | PHE | GLY | GLY | VAL | ||||
| 8 | TYR | ASP | GLN | VAL | LYS | THR | ASN | ASP | LEU | ILE | ||||
| 9 | GLU | TYR | THR | ILE | GLY | ASP | GLY | ARG | LYS | VAL | ||||
| 10 | ARG | ILE | VAL | PHE | THR | HIS | THR | GLY | ASP | THR | ||||
| 11 | THR | ASN | ILE | VAL | GLU | SER | PHE | ASP | PRO | GLU | ||||
| 12 | GLU | THR | ASN | PRO | ARG | GLU | LEU | GLN | GLN | SER | ||||
| 13 | GLY | TRP | GLN | ALA | ILE | LEU | ASN | SER | PHE | LYS | ||||
| 14 | SER | TYR | THR | GLU | ASN | ASN | LEU | GLU | HIS | HIS | ||||
| 15 | HIS | HIS | HIS | HIS |
Samples:
sample_1: Chr148 1 mM; sodium azide 3 mM; DTT 10 mM; calcium chloride 5 mM; sodium chloride 200 mM; MES 20 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.3 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| NH-Jmod | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts