BMRB Entry 17433
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17433
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Title: Structure of the Integrin beta3(A711P,K716A) Transmembrane Segment PubMed: 22178926
Deposition date: 2011-01-27 Original release date: 2011-12-20
Authors: Schmidt, Thomas; Ulmer, Tobias
Citation: Kim, Chungho; Schmidt, Thomas; Cho, Eun-Gyung; Ye, Feng; Ulmer, Tobias; Ginsberg, Mark. "Basic amino-acid side chains regulate transmembrane integrin signalling." Nature 481, 209-213 (2012).
Assembly members:
Integrin_beta3, polymer, 43 residues, 4680.770 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Integrin_beta3: PESPKGPDILVVLLSVMGAI
LLIGLAPLLIWALLITIHDR
KEF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 118 |
| 15N chemical shifts | 38 |
| 1H chemical shifts | 38 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Integrin_beta3 | 1 |
Entities:
Entity 1, Integrin_beta3 43 residues - 4680.770 Da.
| 1 | PRO | GLU | SER | PRO | LYS | GLY | PRO | ASP | ILE | LEU | ||||
| 2 | VAL | VAL | LEU | LEU | SER | VAL | MET | GLY | ALA | ILE | ||||
| 3 | LEU | LEU | ILE | GLY | LEU | ALA | PRO | LEU | LEU | ILE | ||||
| 4 | TRP | ALA | LEU | LEU | ILE | THR | ILE | HIS | ASP | ARG | ||||
| 5 | LYS | GLU | PHE |
Samples:
sample_1: entity, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; DHPC 350 mM; POPC 105 mM; HEPES 25 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.025 M; pH: 7.4; pressure: 1 atm; temperature: 308.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| triple resonance | sample_1 | isotropic | sample_conditions_1 |
| quant j correlation | sample_1 | isotropic | sample_conditions_1 |
| quant j correlation | sample_1 | anisotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB |
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