BMRB Entry 17478
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17478
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Title: Structure of cIAP1 CARD PubMed: 21549626
Deposition date: 2011-02-21 Original release date: 2011-05-12
Authors: Hinds, Mark; Rautureau, Gilles; Day, Catherine
Citation: Lopez, Juanita; John, Sidonie Wicky; Tenev, Tencho; Rautureau, Gilles; Hinds, Mark; Francalanci, Floriana; Wilson, Rebecca; Broemer, Meike; Santoro, Massimo; Day, Catherine; Meier, Pascal. "CARD-Mediated Autoinhibition of cIAP1's E3 Ligase Activity Suppresses Cell Proliferation and Migration." Mol. Cell 42, 569-583 (2011).
Assembly members:
cIAP1-CARD, polymer, 132 residues, 15147.519 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cIAP1-CARD: GSHMLNAEDEKREEEKEKQA
EEMASDDLSLIRKNRMALFQ
QLTCVLPILDNLLKANVINK
QEHDIIKQKTQIPLQARELI
DTILVKGNAAANIFKNCLKE
IDSTLYKNLFVDKNMKYIPT
EDVSGLSLEEQL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 156 |
| 15N chemical shifts | 149 |
| 1H chemical shifts | 165 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cIAP1 CARD | 1 |
Entities:
Entity 1, cIAP1 CARD 132 residues - 15147.519 Da.
residues 435-562 of BIRC2_HUMAN (uniprot) with 4 N-terminal vector derived residues residues, numbered 431-434
| 1 | GLY | SER | HIS | MET | LEU | ASN | ALA | GLU | ASP | GLU | ||||
| 2 | LYS | ARG | GLU | GLU | GLU | LYS | GLU | LYS | GLN | ALA | ||||
| 3 | GLU | GLU | MET | ALA | SER | ASP | ASP | LEU | SER | LEU | ||||
| 4 | ILE | ARG | LYS | ASN | ARG | MET | ALA | LEU | PHE | GLN | ||||
| 5 | GLN | LEU | THR | CYS | VAL | LEU | PRO | ILE | LEU | ASP | ||||
| 6 | ASN | LEU | LEU | LYS | ALA | ASN | VAL | ILE | ASN | LYS | ||||
| 7 | GLN | GLU | HIS | ASP | ILE | ILE | LYS | GLN | LYS | THR | ||||
| 8 | GLN | ILE | PRO | LEU | GLN | ALA | ARG | GLU | LEU | ILE | ||||
| 9 | ASP | THR | ILE | LEU | VAL | LYS | GLY | ASN | ALA | ALA | ||||
| 10 | ALA | ASN | ILE | PHE | LYS | ASN | CYS | LEU | LYS | GLU | ||||
| 11 | ILE | ASP | SER | THR | LEU | TYR | LYS | ASN | LEU | PHE | ||||
| 12 | VAL | ASP | LYS | ASN | MET | LYS | TYR | ILE | PRO | THR | ||||
| 13 | GLU | ASP | VAL | SER | GLY | LEU | SER | LEU | GLU | GLU | ||||
| 14 | GLN | LEU |
Samples:
sample_1: cIAP1-CARD 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 5 mM; H2O 90%; D2O 10%
sample_2: cIAP1-CARD, [U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 5 mM; H2O 90%; D2O 10%
sample_3: cIAP1-CARD, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.140 M; pH: 5.5; pressure: 1 atm; temperature: 303.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
| CC(CO)NH | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_3 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNHB | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_3 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
XEASY, Bartels et al. - data analysis, peak picking
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
- Bruker Avance 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG10600 BAG36235 BAG64288 |
| EMBL | CAH91479 |
| GB | AAC41942 AAC50372 AAC50508 AAH16174 AAH28578 |
| PRF | 2205253A |
| REF | NP_001157 NP_001243092 NP_001243095 NP_001248250 XP_001152534 |
| SP | Q13490 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts