BMRB Entry 17531
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17531
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Title: Three-dimensional structure of the cyclotide Cter M PubMed: 21593408
Deposition date: 2011-03-16 Original release date: 2011-05-27
Authors: Poth, Aaron; Colgrave, Michelle; Lyons, Russell; Daly, Norelle; Craik, David
Citation: Poth, Aaron; Colgrave, Michelle; Lyons, Russell; Daly, Norelle; Craik, David. "From the Cover: Discovery of an unusual biosynthetic origin for circular proteins in legumes." Proc. Natl. Acad. Sci. U.S.A. 108, 10127-10132 (2011).
Assembly members:
entity, polymer, 29 residues, 3084.605 Da.
Natural source: Common Name: Clitoria ternatea Taxonomy ID: 43366 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Clitoria ternatea
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: GLPTCGETCTLGTCYVPDCS
CSWPICMKN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 174 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | circular proteins in legumes | 1 |
Entities:
Entity 1, circular proteins in legumes 29 residues - 3084.605 Da.
| 1 | GLY | LEU | PRO | THR | CYS | GLY | GLU | THR | CYS | THR | ||||
| 2 | LEU | GLY | THR | CYS | TYR | VAL | PRO | ASP | CYS | SER | ||||
| 3 | CYS | SER | TRP | PRO | ILE | CYS | MET | LYS | ASN |
Samples:
sample_1: Cter M 1 mM
sample_conditions_1: ionic strength: . M; pH: 4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
XEASY, Bartels et al. - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker Avance 900 MHz
- Bruker Avance 600 MHz