BMRB Entry 17533
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17533
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Title: Solution structure of the Sex Peptide from Drosophila melanogaster PubMed: 21439282
Deposition date: 2011-03-17 Original release date: 2011-05-03
Authors: Moehle, Kerstin; Freund, Annabelle; Robinson, John
Citation: Moehle, Kerstin; Freund, Annabelle; Kubli, Eric; Robinson, John. "NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster." FEBS Lett. 585, 1197-1202 (2011).
Assembly members:
entity, polymer, 36 residues, 4435.077 Da.
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: WEWPWNRKXTKFXIXSXNXR
DKWCRLNLGPAWGGRC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 77 |
| 15N chemical shifts | 36 |
| 1H chemical shifts | 251 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sex Peptide from Drosophila melanogaster | 1 |
Entities:
Entity 1, Sex Peptide from Drosophila melanogaster 36 residues - 4435.077 Da.
| 1 | TRP | GLU | TRP | PRO | TRP | ASN | ARG | LYS | HYP | THR | ||||
| 2 | LYS | PHE | HYP | ILE | HYP | SER | HYP | ASN | HYP | ARG | ||||
| 3 | ASP | LYS | TRP | CYS | ARG | LEU | ASN | LEU | GLY | PRO | ||||
| 4 | ALA | TRP | GLY | GLY | ARG | CYS |
Samples:
sample_1: TSP0.7 – 3 mM; H2O 90%; D2O 10%
sample_2: TSP0.7 – 3 mM; D2O 100%
sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment, peak picking
TOPSPIN, Bruker Biospin - collection, processing
DYANA, Guntert, Braun and Wuthrich - refinement, structure solution
SPSCAN, Glaser - peak integration
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
Related Database Links:
| PDB |
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