BMRB Entry 17537
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17537
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Title: Solution structure of a Human minimembrane protein OST4 PubMed: 21609714
Deposition date: 2011-03-21 Original release date: 2011-06-03
Authors: Gayen, Shovanlal; Kang, Congbao
Citation: Gayen, Shovanlal; Kang, Congbao. "Solution structure of a human minimembrane protein Ost4, a subunit of the oligosaccharyltransferase complex." Biochem. Biophys. Res. Commun. 409, 572-576 (2011).
Assembly members:
Human minimembrane protein OST4, polymer, 37 residues, 4196.043 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Human minimembrane protein OST4: MITDVQLAIFANMLGVSLFL
LVVLYHYVAVNNPKKQE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 202 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Human minimembrane protein OST4 | 1 |
Entities:
Entity 1, Human minimembrane protein OST4 37 residues - 4196.043 Da.
| 1 | MET | ILE | THR | ASP | VAL | GLN | LEU | ALA | ILE | PHE | ||||
| 2 | ALA | ASN | MET | LEU | GLY | VAL | SER | LEU | PHE | LEU | ||||
| 3 | LEU | VAL | VAL | LEU | TYR | HIS | TYR | VAL | ALA | VAL | ||||
| 4 | ASN | ASN | PRO | LYS | LYS | GLN | GLU |
Samples:
sample_1: OST42 – 3 mM; CDC13 44%; Cd3OH 44%; H2O 12%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, data analysis
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAC24992 BAC25041 BAC25115 BAC25288 BAC25588 |
| GB | AAH02177 AAH82316 AAI57815 ACH44975 ACH44976 |
| REF | NP_001128162 NP_001128163 NP_001128164 NP_001128165 NP_001185794 |
| SP | B0BLS0 P0C6T2 Q99LX8 |
| TPG | DAA24455 |