BMRB Entry 17572

Name: i6A37_tyrASL
Published: 2011-08-02

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PDB ID: 2lbq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17572
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: i6A37_tyrASL PubMed: 21782828

Deposition date: 2011-04-05 Original release date: 2011-08-02

Authors: Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward

Citation: Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward. "Conformation effects of base modification on the anticodon stem-loop of Bacillus subtilis tRNA(Tyr)." J. Mol. Biol. 412, 285-303 (2011).

Assembly members:
i6A37_tyrASL, polymer, 17 residues, 417.358 Da.

Natural source: Common Name: B. subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free

Entity Sequences (FASTA):
i6A37_tyrASL: GGGGACUGUAXAUCCCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	101
15N chemical shifts	24
1H chemical shifts	143

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	i6A37 tyrASL	1

Entities:

Entity 1, i6A37 tyrASL 17 residues - 417.358 Da.

1

G

A

C

U

G

U

A

2

6IA

A

U

C

Samples:

sample_1: i6A tyrASL, [U-100% 13C; U-100% 15N], 2.0 mM; potassium phosphate 10 mM; potassium chloride 10 mM; EDTA 0.02 mM; D2O 100%

sample_conditions_1: pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D HCCH-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C Long Range HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D HCCH-RELAY	sample_1	isotropic	sample_conditions_1
2D HCNC	sample_1	isotropic	sample_conditions_1

Software:

FELIX, Accelrys Software Inc. - data analysis, processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

InsightII, Accelrys Software Inc. - data analysis

VNMRJ, Varian - collection

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz