BMRB Entry 17573
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17573
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Title: Conformation Effects of Base Modification on the Anticodon Stem-loop of Bacillus subtilis tRNATYR PubMed: 21782828
Deposition date: 2011-04-05 Original release date: 2011-08-02
Authors: Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward
Citation: Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward. "Conformation effects of base modification on the anticodon stem-loop of Bacillus subtilis tRNA(Tyr)." J. Mol. Biol. 412, 285-303 (2011).
Assembly members:
coi6y, polymer, 17 residues, 417.358 Da.
Natural source: Common Name: B. subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: enzymatic semisynthesis Host organism: cell free
Entity Sequences (FASTA):
coi6y: GGGGACUGUAXAXCCCC
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 38 |
1H chemical shifts | 75 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | coi6y | 1 |
Entities:
Entity 1, coi6y 17 residues - 417.358 Da.
1 | G | G | G | G | A | C | U | G | U | A | ||||
2 | 6IA | A | PSU | C | C | C | C |
Samples:
sample_1: i6A,PSU-ASL 2.0 mM; potassium chloride 10 mM; EDTA 0.02 mM; potassium phosphate 10 mM; D2O 100%
sample_conditions_1: pH: 6.3; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
Natural abundance 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX, Accelrys Software Inc. - data analysis, processing
InsightII, Accelrys Software Inc. - data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation
VNMRJ, Varian - collection
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz