BMRB Entry 17617
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17617
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Title: IIIS4
Deposition date: 2011-05-02 Original release date: 2011-06-03
Authors: Darbon, Herv; De waar, Michel
Citation: Douzi, Badreddine; Darbon, Herve; De waar, Michel. "structure of S4-3 voltage sensor peptide" J. Biol. Chem. ., .-..
Assembly members:
Peptide, polymer, 28 residues, 3351.292 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Peptide: KDINTIKSLRVLRVLRPLKT
IKRLPKLK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 236 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | S4-3 voltage sensor peptide | 1 |
Entities:
Entity 1, S4-3 voltage sensor peptide 28 residues - 3351.292 Da.
| 1 | LYS | ASP | ILE | ASN | THR | ILE | LYS | SER | LEU | ARG | ||||
| 2 | VAL | LEU | ARG | VAL | LEU | ARG | PRO | LEU | LYS | THR | ||||
| 3 | ILE | LYS | ARG | LEU | PRO | LYS | LEU | LYS |
Samples:
sample_1: Peptide 1 mM; D2O 10 mM; H2O 90 mM; Trifluoroethanol 30%
sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift calculation
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA94765 BAA94766 BAB85611 BAB85612 BAD92704 |
| EMBL | CAA40714 CAA40715 CAA40871 CAA40872 CAA68172 |
| GB | AAA40806 AAA42014 AAA51897 AAA51898 AAB49674 |
| PRF | 1709354A 1709354B |
| REF | NP_000059 NP_000709 NP_001035993 NP_001068597 NP_001095163 |
| SP | O00555 O55017 P27884 P54282 P56699 |
| TPG | DAA28081 |