BMRB Entry 17623
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17623
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Title: MODEL OF HUMAN U2AF65 TANDEM RRM1 AND RRM2 DOMAINS WITH EIGHT-SITE URIDINE BINDING
Deposition date: 2011-05-04 Original release date: 2011-08-03
Authors: Mackereth, C.; Madl, T.; Simon, B.; Zanier, K.; Gasch, A.; Sattler, Michael
Citation: Mackereth, C.; Madl, T.; Bonnal, S.; Simon, B.; Zanier, K.; Gasch, A.; Rybin, V.; Valcarcel, J.; Sattler, Michael. "Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF" Nature ., .-..
Assembly members:
HUMAN_U2AF65, polymer, 198 residues, 21491.534 Da.
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3', polymer, 9 residues, 2774.53744 Da.
Natural source: Common Name: HUMAN Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis Host organism: unknown
Entity Sequences (FASTA):
HUMAN_U2AF65: GAMARRLYVGNIPFGITEEA
MMDFFNAQMRLGGLTQAPGN
PVLAVQINQDKNFAFLEFRS
VDETTQAMAFDGIIFQGQSL
KIRRPHDYQPLPGMSENPSV
YVPGVVSTVVPDSAHKLFIG
GLPNYLNDDQVKELLTSFGP
LKAFNLVKDSATGLSKGYAF
CEYVDINVTDQAIAGLNGMQ
LGDKKLLVQRASVGAKNA
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3': UUUUUUUUU
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 375 |
| 15N chemical shifts | 184 |
| 1H chemical shifts | 914 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HUMAN U2AF65 | 1 |
| 2 | 5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3' | 2 |
Entities:
Entity 1, HUMAN U2AF65 198 residues - 21491.534 Da.
| 1 | GLY | ALA | MET | ALA | ARG | ARG | LEU | TYR | VAL | GLY | ||||
| 2 | ASN | ILE | PRO | PHE | GLY | ILE | THR | GLU | GLU | ALA | ||||
| 3 | MET | MET | ASP | PHE | PHE | ASN | ALA | GLN | MET | ARG | ||||
| 4 | LEU | GLY | GLY | LEU | THR | GLN | ALA | PRO | GLY | ASN | ||||
| 5 | PRO | VAL | LEU | ALA | VAL | GLN | ILE | ASN | GLN | ASP | ||||
| 6 | LYS | ASN | PHE | ALA | PHE | LEU | GLU | PHE | ARG | SER | ||||
| 7 | VAL | ASP | GLU | THR | THR | GLN | ALA | MET | ALA | PHE | ||||
| 8 | ASP | GLY | ILE | ILE | PHE | GLN | GLY | GLN | SER | LEU | ||||
| 9 | LYS | ILE | ARG | ARG | PRO | HIS | ASP | TYR | GLN | PRO | ||||
| 10 | LEU | PRO | GLY | MET | SER | GLU | ASN | PRO | SER | VAL | ||||
| 11 | TYR | VAL | PRO | GLY | VAL | VAL | SER | THR | VAL | VAL | ||||
| 12 | PRO | ASP | SER | ALA | HIS | LYS | LEU | PHE | ILE | GLY | ||||
| 13 | GLY | LEU | PRO | ASN | TYR | LEU | ASN | ASP | ASP | GLN | ||||
| 14 | VAL | LYS | GLU | LEU | LEU | THR | SER | PHE | GLY | PRO | ||||
| 15 | LEU | LYS | ALA | PHE | ASN | LEU | VAL | LYS | ASP | SER | ||||
| 16 | ALA | THR | GLY | LEU | SER | LYS | GLY | TYR | ALA | PHE | ||||
| 17 | CYS | GLU | TYR | VAL | ASP | ILE | ASN | VAL | THR | ASP | ||||
| 18 | GLN | ALA | ILE | ALA | GLY | LEU | ASN | GLY | MET | GLN | ||||
| 19 | LEU | GLY | ASP | LYS | LYS | LEU | LEU | VAL | GLN | ARG | ||||
| 20 | ALA | SER | VAL | GLY | ALA | LYS | ASN | ALA |
Entity 2, 5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP)-3' 9 residues - 2774.53744 Da.
| 1 | U | U | U | U | U | U | U | U | U |
Samples:
sample_1: HUMAN_U2AF65, [U-13C; U-15N], 0.2 mM; RNA 0.24 mM; H2O 90%; D2O 10%; sodium phosphate 20 mM; NaCl 50 mM
sample_conditions_1: ionic strength: 70.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 295.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H | sample_1 | solution | sample_conditions_1 |
| 15N HSQC (PRE) | sample_1 | solution | sample_conditions_1 |
| 15N TROSY (HN-N RDC) | sample_1 | solution | sample_conditions_1 |
| 3D HNCO (N-CO RDC) | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - collection
CNS vany, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - chemical shift assignment
SPARKY vany, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| UNP | U2AF2_HUMAN |
| BMRB | 17622 |
| PDB | |
| DBJ | BAC37309 BAG70075 BAG70201 BAI45669 |
| EMBL | CAA45409 CAA45874 CAA45875 CAF97922 |
| GB | AAH07487 AAH08740 AAH30574 AAH43071 AAH44032 |
| PRF | 1805352A |
| REF | NP_001001217 NP_001012496 NP_001025127 NP_001068804 NP_001080595 |
| SP | P26368 P26369 |
| TPG | DAA19368 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts