BMRB Entry 17635
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17635
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Title: solution structure of FUS/TLS RRM domain
Deposition date: 2011-05-10 Original release date: 2012-06-04
Authors: Liu, Xuehui; Ren, Jintao; Niu, Chunyan; Gong, Weimin; Feng, Wei
Citation: Liu, Xuehui; Ren, Jintao; Niu, Chunyan; Feng, Wei; Gong, Weimin. "TLS-RRM is a promiscuous nucleic acid binding domain" Not known ., .-..
Assembly members:
FUS/TLS RRM domain, polymer, 108 residues, 11747.013 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
FUS/TLS RRM domain: EQDNSDNNTIFVQGLGENVT
IESVADYFKQIGIIKTNKKT
GQPMINLYTDRETGKLKGEA
TVSFDDPPSAKAAIDWFDGK
EFSGNPIKVSFATRRADFNR
GGGNGRGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 446 |
| 15N chemical shifts | 117 |
| 1H chemical shifts | 657 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FUS/TLS RRM domain | 1 |
Entities:
Entity 1, FUS/TLS RRM domain 108 residues - 11747.013 Da.
| 1 | GLU | GLN | ASP | ASN | SER | ASP | ASN | ASN | THR | ILE | ||||
| 2 | PHE | VAL | GLN | GLY | LEU | GLY | GLU | ASN | VAL | THR | ||||
| 3 | ILE | GLU | SER | VAL | ALA | ASP | TYR | PHE | LYS | GLN | ||||
| 4 | ILE | GLY | ILE | ILE | LYS | THR | ASN | LYS | LYS | THR | ||||
| 5 | GLY | GLN | PRO | MET | ILE | ASN | LEU | TYR | THR | ASP | ||||
| 6 | ARG | GLU | THR | GLY | LYS | LEU | LYS | GLY | GLU | ALA | ||||
| 7 | THR | VAL | SER | PHE | ASP | ASP | PRO | PRO | SER | ALA | ||||
| 8 | LYS | ALA | ALA | ILE | ASP | TRP | PHE | ASP | GLY | LYS | ||||
| 9 | GLU | PHE | SER | GLY | ASN | PRO | ILE | LYS | VAL | SER | ||||
| 10 | PHE | ALA | THR | ARG | ARG | ALA | ASP | PHE | ASN | ARG | ||||
| 11 | GLY | GLY | GLY | ASN | GLY | ARG | GLY | GLY |
Samples:
sample_1: DSS 0.5 mM; Tris 20 mM; NaCl 50 mM; H2O 10%; D2O, [U-100% 2H], 90%; NaN3 0.02%; FUS_TLS_RRM_domain, [U-100% 15N; U-100% 13C], 1 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92139 BAE24196 BAE26524 BAE26972 BAE87186 |
| EMBL | CAA50559 CAG33028 CAJ83479 |
| GB | AAA79948 AAB27102 AAC13543 AAC35284 AAC35285 |
| PRF | 1916411B |
| REF | NP_001001531 NP_001012137 NP_001080383 NP_001086293 NP_001090836 |
| SP | P35637 P56959 Q28009 |
| TPG | DAA15352 |
Download simulated HSQC data in one of the following formats:
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