BMRB Entry 17667
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17667
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 13C & 13N chemical shift assignments for Toxoplasma gondii Microneme protein 4 (MIC4) residues 410-491 (apple domain 5)
Deposition date: 2011-05-25 Original release date: 2011-06-24
Authors: Matthews, Steve; Pete, Simpson; Jan, Marchant
Citation: Cowper, Ben. "TgMIC4-apple5 assignments" Not known ., .-..
Assembly members:
TgMIC4-apple5, polymer, 82 residues, Formula weight is not available
Natural source: Common Name: Toxoplasma gondii Taxonomy ID: 5811 Superkingdom: Eukaryota Kingdom: not available Genus/species: Toxoplasma gondii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TgMIC4-apple5: SPDFHDEVECVHTGNIGSKA
QTIGEVKRASSLSECRARCQ
AEKECSHYTYNVKSGLCYPK
RGKPQFYKYLGDMTGSRTCD
TS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 315 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 452 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TgMIC4-apple5 | 1 |
Entities:
Entity 1, TgMIC4-apple5 82 residues - Formula weight is not available
| 1 | SER | PRO | ASP | PHE | HIS | ASP | GLU | VAL | GLU | CYS | ||||
| 2 | VAL | HIS | THR | GLY | ASN | ILE | GLY | SER | LYS | ALA | ||||
| 3 | GLN | THR | ILE | GLY | GLU | VAL | LYS | ARG | ALA | SER | ||||
| 4 | SER | LEU | SER | GLU | CYS | ARG | ALA | ARG | CYS | GLN | ||||
| 5 | ALA | GLU | LYS | GLU | CYS | SER | HIS | TYR | THR | TYR | ||||
| 6 | ASN | VAL | LYS | SER | GLY | LEU | CYS | TYR | PRO | LYS | ||||
| 7 | ARG | GLY | LYS | PRO | GLN | PHE | TYR | LYS | TYR | LEU | ||||
| 8 | GLY | ASP | MET | THR | GLY | SER | ARG | THR | CYS | ASP | ||||
| 9 | THR | SER |
Samples:
sample_1: TgMIC4-apple5, [U-98% 13C; U-98% 15N], 100 – 200 mM; potassium phosphate 50 mM; sodium chloride 100 mM; H20 90%; D20 10%
sample_2: TgMIC4-apple5, [U-98% 13C; U-98% 15N], 100-200 mM; potassium phosphate 50 mM; sodium chloride 100 mM; H20 90%; D20 10%
sample_conditions_1: ionic strength: 0.7 M; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts