BMRB Entry 17668
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17668
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Title: NMR solution structure of ZiaAN sub mutant PubMed: 22198771
Deposition date: 2011-05-26 Original release date: 2011-11-22
Authors: Banci, Lucia; Bertini, Ivano; Felli, Isabella; Pavelkova, Anna
Citation: Tottey, Steve; Patterson, Carl; Banci, Lucia; Bertini, Ivano; Felli, Isabella; Pavelkova, Anna; Dainty, Samantha; Pernil, Rafael; Waldron, Kevin; Foster, Andrew; Robinson, Nigel. "Cyanobacterial metallochaperone inhibits deleterious side reactions of copper." Proc. Natl. Acad. Sci. U.S.A. 109, 95-100 (2012).
Assembly members:
ZiaAn sub mutant, polymer, 106 residues, 7586.802 Da.
Natural source: Common Name: Synechocystis sp. PCC 6803 Taxonomy ID: 1148 Superkingdom: Bacteria Kingdom: not available Genus/species: Synechocystis sp.
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ZiaAn sub mutant: PLKTQQMQVGGMRCAACASS
IERALERLKGVAEASVTVAT
GRLTVTYDPKQVSEITIQER
IAALGYTLAEPKSSVTLNGH
KHPHSHREEGHSHSHGAGEF
NLKQEL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 403 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 512 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ZiaAn sub mutant | 1 |
Entities:
Entity 1, ZiaAn sub mutant 106 residues - 7586.802 Da.
| 1 | PRO | LEU | LYS | THR | GLN | GLN | MET | GLN | VAL | GLY | ||||
| 2 | GLY | MET | ARG | CYS | ALA | ALA | CYS | ALA | SER | SER | ||||
| 3 | ILE | GLU | ARG | ALA | LEU | GLU | ARG | LEU | LYS | GLY | ||||
| 4 | VAL | ALA | GLU | ALA | SER | VAL | THR | VAL | ALA | THR | ||||
| 5 | GLY | ARG | LEU | THR | VAL | THR | TYR | ASP | PRO | LYS | ||||
| 6 | GLN | VAL | SER | GLU | ILE | THR | ILE | GLN | GLU | ARG | ||||
| 7 | ILE | ALA | ALA | LEU | GLY | TYR | THR | LEU | ALA | GLU | ||||
| 8 | PRO | LYS | SER | SER | VAL | THR | LEU | ASN | GLY | HIS | ||||
| 9 | LYS | HIS | PRO | HIS | SER | HIS | ARG | GLU | GLU | GLY | ||||
| 10 | HIS | SER | HIS | SER | HIS | GLY | ALA | GLY | GLU | PHE | ||||
| 11 | ASN | LEU | LYS | GLN | GLU | LEU |
Samples:
sample_1: ZiaAN sub, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 50 mM; H20 90%; D20 10%
sample_conditions_1: ionic strength: 50 mM; pH: 7.00; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
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