BMRB Entry 17691
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17691
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the HMG box DNA-binding domain of human stem cell transcription factor Sox2
Deposition date: 2011-06-06 Original release date: 2011-07-06
Authors: Sahu, Sarata; Markley, John; Tonelli, Marco; Bahrami, Arash; Eghbalnia, Hamid
Citation: Sahu, Sarata; Markley, John; Tonelli, Marco; Bahrami, Arash; Eghbalnia, Hamid. "Not known" Not known ., .-..
Assembly members:
Sox2, polymer, 81 residues, 9920.689 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Sox2: SDRVKRPMNAFMVWSRGQRR
KMAQENPKMHNSEISKRLGA
EWKLLSETEKRPFIDEAKRL
RALHMKEHPDYKYRPRRKTK
T
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 370 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 532 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sox2 | 1 |
Entities:
Entity 1, Sox2 81 residues - 9920.689 Da.
| 1 | SER | ASP | ARG | VAL | LYS | ARG | PRO | MET | ASN | ALA | ||||
| 2 | PHE | MET | VAL | TRP | SER | ARG | GLY | GLN | ARG | ARG | ||||
| 3 | LYS | MET | ALA | GLN | GLU | ASN | PRO | LYS | MET | HIS | ||||
| 4 | ASN | SER | GLU | ILE | SER | LYS | ARG | LEU | GLY | ALA | ||||
| 5 | GLU | TRP | LYS | LEU | LEU | SER | GLU | THR | GLU | LYS | ||||
| 6 | ARG | PRO | PHE | ILE | ASP | GLU | ALA | LYS | ARG | LEU | ||||
| 7 | ARG | ALA | LEU | HIS | MET | LYS | GLU | HIS | PRO | ASP | ||||
| 8 | TYR | LYS | TYR | ARG | PRO | ARG | ARG | LYS | THR | LYS | ||||
| 9 | THR |
Samples:
sample_1: Sox2, [U-100% 13C; U-100% 15N], 0.7 ± 0.02 mM; DTT 5 ± 0.05 %; MOPS 10 mM; NaCl 100 mM; H20 95%; D20 5%
sample_conditions_1: ionic strength: 100 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_15N-separated_NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY (AROMATIC) | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA09168 BAC55017 BAC67545 BAC75668 BAE20882 |
| EMBL | CAA63847 CAA65725 CAA80631 CAA83435 CAF91617 |
| GB | AAA35997 AAB09662 AAB62821 AAC14215 AAC31791 |
| PRF | 1612347C |
| REF | NP_001081691 NP_001098933 NP_001102651 NP_001116669 NP_001135190 |
| SP | O42569 P48430 P48431 P48432 P54231 |
| TPG | DAA33313 |