BMRB Entry 17719
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17719
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Title: Solution structure of esophageal cancer-related gene 2 PubMed: 22528291
Deposition date: 2011-06-20 Original release date: 2012-05-09
Authors: Feng, Yingang; Geng, Yong; Wang, Jinfeng
Citation: Feng, Yingang; Geng, Yong; Zhou, Tao; Wang, Jinfeng. "NMR structure note: human esophageal cancer-related gene 2." J. Biomol. NMR 53, 65-70 (2012).
Assembly members:
esophageal cancer-related gene 2, polymer, 66 residues, 7209.305 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
esophageal cancer-related gene 2: SEAASLSPKKVDCSIYKKYP
VVAIPCPITYLPVCGSDYIT
YGNECHLCTESLKSNGRVQF
LHDGSC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 289 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 446 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | esophageal cancer-related gene 2 | 1 |
Entities:
Entity 1, esophageal cancer-related gene 2 66 residues - 7209.305 Da.
| 1 | SER | GLU | ALA | ALA | SER | LEU | SER | PRO | LYS | LYS | ||||
| 2 | VAL | ASP | CYS | SER | ILE | TYR | LYS | LYS | TYR | PRO | ||||
| 3 | VAL | VAL | ALA | ILE | PRO | CYS | PRO | ILE | THR | TYR | ||||
| 4 | LEU | PRO | VAL | CYS | GLY | SER | ASP | TYR | ILE | THR | ||||
| 5 | TYR | GLY | ASN | GLU | CYS | HIS | LEU | CYS | THR | GLU | ||||
| 6 | SER | LEU | LYS | SER | ASN | GLY | ARG | VAL | GLN | PHE | ||||
| 7 | LEU | HIS | ASP | GLY | SER | CYS |
Samples:
sample_1: sodium acetate, [U-2H], 50 mM; potassium chloride 100 mM; DSS 0.01%; sodium azide 0.01%; EC-RG2, [U-13C; U-15N], 0.5 – 1.0 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 5.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
FELIX, Accelrys Software Inc. - chemical shift assignment, data analysis, refinement
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| GB | AAI09386 AAI10068 AAK27795 AAQ89431 AIC52595 |
| REF | NP_115955 XP_001103960 XP_001160972 XP_002816126 XP_003266657 |
| SP | P58062 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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