BMRB Entry 17745
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17745
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Title: NMR Assignment of PFV RNase H domain PubMed: 22325739
Deposition date: 2011-06-29 Original release date: 2012-03-27
Authors: Leo, Berit; Schweimer, Kristian; Woehrl, Birgitta
Citation: Leo, Berit; Hartl, Maximilian; Schweimer, Kristian; Mayr, Florian; Wohrl, Birgitta. "Insights into the structure and activity of prototype foamy virus RNase H." Retrovirology 9, 14-14 (2012).
Assembly members:
PFV_RNase_H, polymer, 165 residues, Formula weight is not available
Natural source: Common Name: prototype foamy virus Taxonomy ID: not available Superkingdom: Viruses Kingdom: not available Genus/species: foamy virus prototype foamy virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PFV_RNase_H: GAMGQYEGVFYTDGSAIKSP
DPTKSNNAGMGIVHATYKPE
YQVLNQWSIPLGNHTAQMAE
IAAVEFACKKALKIPGPVLV
ITDSFYVAESANKELPYWKS
NGFVNNKKKPLKHISKWKSI
AECLSMKPDITIQHEKGHQP
TNTSIHTEGNALADKLATQG
SYVVN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 600 |
| 15N chemical shifts | 142 |
| 1H chemical shifts | 1006 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PFV RNase H | 1 |
Entities:
Entity 1, PFV RNase H 165 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | GLN | TYR | GLU | GLY | VAL | PHE | ||||
| 2 | TYR | THR | ASP | GLY | SER | ALA | ILE | LYS | SER | PRO | ||||
| 3 | ASP | PRO | THR | LYS | SER | ASN | ASN | ALA | GLY | MET | ||||
| 4 | GLY | ILE | VAL | HIS | ALA | THR | TYR | LYS | PRO | GLU | ||||
| 5 | TYR | GLN | VAL | LEU | ASN | GLN | TRP | SER | ILE | PRO | ||||
| 6 | LEU | GLY | ASN | HIS | THR | ALA | GLN | MET | ALA | GLU | ||||
| 7 | ILE | ALA | ALA | VAL | GLU | PHE | ALA | CYS | LYS | LYS | ||||
| 8 | ALA | LEU | LYS | ILE | PRO | GLY | PRO | VAL | LEU | VAL | ||||
| 9 | ILE | THR | ASP | SER | PHE | TYR | VAL | ALA | GLU | SER | ||||
| 10 | ALA | ASN | LYS | GLU | LEU | PRO | TYR | TRP | LYS | SER | ||||
| 11 | ASN | GLY | PHE | VAL | ASN | ASN | LYS | LYS | LYS | PRO | ||||
| 12 | LEU | LYS | HIS | ILE | SER | LYS | TRP | LYS | SER | ILE | ||||
| 13 | ALA | GLU | CYS | LEU | SER | MET | LYS | PRO | ASP | ILE | ||||
| 14 | THR | ILE | GLN | HIS | GLU | LYS | GLY | HIS | GLN | PRO | ||||
| 15 | THR | ASN | THR | SER | ILE | HIS | THR | GLU | GLY | ASN | ||||
| 16 | ALA | LEU | ALA | ASP | LYS | LEU | ALA | THR | GLN | GLY | ||||
| 17 | SER | TYR | VAL | VAL | ASN |
Samples:
sample_1: PFV RNase H, [U-13C; U-15N], 1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts