BMRB Entry 17833
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17833
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Title: Skint1 IgV
Deposition date: 2011-08-05 Original release date: 2016-02-29
Authors: Salim, Mahboob; Knowles, Timothy; Willcox, Carrie; Mohammed, Fiyaz; Woodard, Martin; Overduin, Michael; Hayday, Adrian; Willcox, Benjamin
Citation: Salim, Mahboob; Knowles, Timothy; Willcox, Carrie; Mohammed, Fiyaz; Woodard, Martin; Overduin, Michael; Hayday, Adrian; Willcox, Benjamin. "Characterisation of a putative receptor binding surface on Skint-1, a critical determinant of dendritic epidermal T cell selection" J. Biol. Chem. ., .-. (2016).
Assembly members:
Skint1_IgV, polymer, 238 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Skint1_IgV: MSSEPFIVNGLEGPVLASLG
GNLELSCQLSPPQQAQHMEI
RWFRNLYTEPVHLYRDGKDM
FGEIISKYVERTELLKDGIG
EGKVTLRIFNVTVDDDGSYH
CVFKDGDFYEEHITEVKITM
SSEPFIVNGLEGPVLASLGG
NLELSCQLSPPQQAQHMEIR
WFRNLYTEPVHLYRDGKDMF
GEIISKYVERTELLKDGIGE
GKVTLRIFNVTVDDDGSYHC
VFKDGDFYEEHITEVKIT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 424 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 641 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Skint1 IgV | 1 |
Entities:
Entity 1, Skint1 IgV 238 residues - Formula weight is not available
2 to 119 is the distal membrane Ig variable domain
| 1 | MET | SER | SER | GLU | PRO | PHE | ILE | VAL | ASN | GLY | ||||
| 2 | LEU | GLU | GLY | PRO | VAL | LEU | ALA | SER | LEU | GLY | ||||
| 3 | GLY | ASN | LEU | GLU | LEU | SER | CYS | GLN | LEU | SER | ||||
| 4 | PRO | PRO | GLN | GLN | ALA | GLN | HIS | MET | GLU | ILE | ||||
| 5 | ARG | TRP | PHE | ARG | ASN | LEU | TYR | THR | GLU | PRO | ||||
| 6 | VAL | HIS | LEU | TYR | ARG | ASP | GLY | LYS | ASP | MET | ||||
| 7 | PHE | GLY | GLU | ILE | ILE | SER | LYS | TYR | VAL | GLU | ||||
| 8 | ARG | THR | GLU | LEU | LEU | LYS | ASP | GLY | ILE | GLY | ||||
| 9 | GLU | GLY | LYS | VAL | THR | LEU | ARG | ILE | PHE | ASN | ||||
| 10 | VAL | THR | VAL | ASP | ASP | ASP | GLY | SER | TYR | HIS | ||||
| 11 | CYS | VAL | PHE | LYS | ASP | GLY | ASP | PHE | TYR | GLU | ||||
| 12 | GLU | HIS | ILE | THR | GLU | VAL | LYS | ILE | THR | MET | ||||
| 13 | SER | SER | GLU | PRO | PHE | ILE | VAL | ASN | GLY | LEU | ||||
| 14 | GLU | GLY | PRO | VAL | LEU | ALA | SER | LEU | GLY | GLY | ||||
| 15 | ASN | LEU | GLU | LEU | SER | CYS | GLN | LEU | SER | PRO | ||||
| 16 | PRO | GLN | GLN | ALA | GLN | HIS | MET | GLU | ILE | ARG | ||||
| 17 | TRP | PHE | ARG | ASN | LEU | TYR | THR | GLU | PRO | VAL | ||||
| 18 | HIS | LEU | TYR | ARG | ASP | GLY | LYS | ASP | MET | PHE | ||||
| 19 | GLY | GLU | ILE | ILE | SER | LYS | TYR | VAL | GLU | ARG | ||||
| 20 | THR | GLU | LEU | LEU | LYS | ASP | GLY | ILE | GLY | GLU | ||||
| 21 | GLY | LYS | VAL | THR | LEU | ARG | ILE | PHE | ASN | VAL | ||||
| 22 | THR | VAL | ASP | ASP | ASP | GLY | SER | TYR | HIS | CYS | ||||
| 23 | VAL | PHE | LYS | ASP | GLY | ASP | PHE | TYR | GLU | GLU | ||||
| 24 | HIS | ILE | THR | GLU | VAL | LYS | ILE | THR |
Samples:
sample_1: Skint1 IgV, [U-100% 13C; U-100% 15N], 1.4 mM; MES 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts