BMRB Entry 17888

Name: NMR Structure of the Polyserine Tract of Apis mellifera Vitellogenin, residues 358-392
Published: 2012-08-17

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PDB ID: 2lic
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17888
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR Structure of the Polyserine Tract of Apis mellifera Vitellogenin, residues 358-392 PubMed: 22573762

Deposition date: 2011-08-29 Original release date: 2012-08-17

Authors: Halskau, Heli; Halskau, Oyvind

Citation: Havukainen, Heli; Underhaug, Jarl; Wolschin, Florian; Amdam, Gro; Halskau, Oyvind. "A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation" J. Exp. Biol. 215, 1837-1846 (2012).

Assembly members:
Vg, polymer, 35 residues, 3986.349 Da.

Natural source: Common Name: honey bee Taxonomy ID: 7460 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Apis mellifera

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
Vg: EKLKQDILNLRTDISTSXSS ISSSEENDFWQPKPT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	223

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Polyserine Tract of Apis mellifera Vitellogenin	1

Entities:

Entity 1, Polyserine Tract of Apis mellifera Vitellogenin 35 residues - 3986.349 Da.

1

GLU

LYS

LEU

LYS

GLN

ASP

ILE

LEU

ASN

LEU

2

ARG

THR

ASP

ILE

SER

THR

SER

SEP

SER

3

ILE

SER

GLU

ASN

ASP

PHE

TRP

4

GLN

PRO

LYS

PRO

THR

Samples:

sample_1: Vg, [U-15N]-ILE, 7.8 w/v; sodium azide 0.02%; sodium phosphate 50 mM; DSS 5%

sample_conditions_1: ionic strength: 0.06 M; pH: 6.7; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

ARIA v1.2, Linge, O, . - data analysis

NMR spectrometers:

Bruker AV600 600 MHz

Biological Magnetic Resonance Data Bank