BMRB Entry 17914
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17914
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Title: Integral membrane core domain of the mercury transporter MerF in lipid bilayer membranes PubMed: 22217388
Deposition date: 2011-09-03 Original release date: 2012-01-17
Authors: Das, Bibhuti; Nothnagel, Henry; Lu, George; Son, Woo Sung; Park, Sang Ho; Tian, Ye; Marassi, Francesca; Opella, Stanley
Citation: Das, Bibhuti; Nothnagel, Henry; Lu, George; Son, Woo Sung; Tian, Ye; Marassi, Francesca; Opella, Stanley. "Structure determination of a membrane protein in proteoliposomes." J. Am. Chem. Soc. 134, 2047-2056 (2012).
Assembly members:
MerFt, polymer, 60 residues, 6281.516 Da.
ANI, non-polymer, 161.124 Da.
Natural source: Common Name: Morganella morganii Taxonomy ID: 582 Superkingdom: Bacteria Kingdom: not available Genus/species: Morganella morganii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MerFt: IGTTLVALSSFTPVLVILLG
VVGLSALTGYLDYVLLPALA
IFIGLTIYAIQRKRQADASS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 110 |
| 15N chemical shifts | 55 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MerFt | 1 |
| 2 | ANI | 2 |
Entities:
Entity 1, MerFt 60 residues - 6281.516 Da.
| 1 | ILE | GLY | THR | THR | LEU | VAL | ALA | LEU | SER | SER | |
| 2 | PHE | THR | PRO | VAL | LEU | VAL | ILE | LEU | LEU | GLY | |
| 3 | VAL | VAL | GLY | LEU | SER | ALA | LEU | THR | GLY | TYR | |
| 4 | LEU | ASP | TYR | VAL | LEU | LEU | PRO | ALA | LEU | ALA | |
| 5 | ILE | PHE | ILE | GLY | LEU | THR | ILE | TYR | ALA | ILE | |
| 6 | GLN | ARG | LYS | ARG | GLN | ALA | ASP | ALA | SER | SER |
Entity 2, ANI - C7 H6 F3 N - 161.124 Da.
| 1 | ANI |
Samples:
sample_1: MerFt, [U-100% 13C; U-100% 15N], 3.6 mg; H2O 100.0%
sample_conditions_1: ionic strength: 10 mM; pH: 6.1; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| see publication | sample_1 | anisotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMR spectrometers:
- Bruker Avance 750 MHz