BMRB Entry 17958
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17958
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Title: C9L,C14L-LeuA PubMed: 22121114
Deposition date: 2011-09-23 Original release date: 2012-01-09
Authors: Sit, Clarissa; Lohans, Christopher; van Belkum, Marco; Campbell, Chantel; Miskolzie, Mark; Vederas, John
Citation: Sit, Clarissa; Lohans, Christopher; van Belkum, Marco; Campbell, Chantel; Miskolzie, Mark; Vederas, John. "Substitution of a Conserved Disulfide in the Type IIa Bacteriocin, Leucocin A, with L-Leucine and L-Serine Residues: Effects on Activity and Three-Dimensional Structure" Chembiochem 13, 35-38 (2012).
Assembly members:
entity, polymer, 37 residues, 3957.392 Da.
Natural source: Common Name: Leuconostoc gelidum Taxonomy ID: 1244 Superkingdom: Bacteria Kingdom: not available Genus/species: Leuconostoc gelidum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: KYYGNGVHLTKSGLSVNWGE
AFSAGVHRLANGGNGFW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 126 |
| 15N chemical shifts | 36 |
| 1H chemical shifts | 194 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C9L,C14L-LeuA | 1 |
Entities:
Entity 1, C9L,C14L-LeuA 37 residues - 3957.392 Da.
| 1 | LYS | TYR | TYR | GLY | ASN | GLY | VAL | HIS | LEU | THR | ||||
| 2 | LYS | SER | GLY | LEU | SER | VAL | ASN | TRP | GLY | GLU | ||||
| 3 | ALA | PHE | SER | ALA | GLY | VAL | HIS | ARG | LEU | ALA | ||||
| 4 | ASN | GLY | GLY | ASN | GLY | PHE | TRP |
Samples:
sample_1: C9L,C14L-LeuA, [U-13C; U-15N], 0.6 mM; TFE, [U-2H], 90%; H2O 10%; TFA 0.1%; DSS 2.6 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Varian VNMRS 700 MHz
Related Database Links:
| PDB |
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