BMRB Entry 18008
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18008
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Title: TBA PubMed: 22101937
Deposition date: 2011-10-18 Original release date: 2011-11-02
Authors: Tripsianes, Konstantinos; Madl, T.; Machyna, M.; Fessas, D.; Englbrecht, C.; Neugebauer, U.; Sattler, M.
Citation: Tripsianes, Konstantinos; Madl, T.; Machyna, M.; Fessas, D.; Englbrecht, C.; Fischer, U.; Neugebauer, K.; Sattler, M.. "Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins" Nat. Struct. Mol. Biol. 18, 1414-1420 (2011).
Assembly members:
A, polymer, 64 residues, 6921.46 Da.
DA2, non-polymer, 202.254 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
A: ASTQPTHSWKVGDKCMAVWS
EDGQCYEAEIEEIDEENGTA
AITFAGYGNAEVTPLLNLKP
VEEG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 200 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 435 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein | 1 |
| 2 | DA2 | 2 |
Entities:
Entity 1, protein 64 residues - 6921.46 Da.
| 1 | ALA | SER | THR | GLN | PRO | THR | HIS | SER | TRP | LYS | ||||
| 2 | VAL | GLY | ASP | LYS | CYS | MET | ALA | VAL | TRP | SER | ||||
| 3 | GLU | ASP | GLY | GLN | CYS | TYR | GLU | ALA | GLU | ILE | ||||
| 4 | GLU | GLU | ILE | ASP | GLU | GLU | ASN | GLY | THR | ALA | ||||
| 5 | ALA | ILE | THR | PHE | ALA | GLY | TYR | GLY | ASN | ALA | ||||
| 6 | GLU | VAL | THR | PRO | LEU | LEU | ASN | LEU | LYS | PRO | ||||
| 7 | VAL | GLU | GLU | GLY |
Entity 2, DA2 - C8 H18 N4 O2 - 202.254 Da.
| 1 | DA2 |
Samples:
sample_new_1: SPF30, [U-13C; U-15N], 4 mM; DA2 18 mM; H2O 93%; D2O 7%; phosphate 20 mM; NaCl 50 mM
sample_conditions_new_1: ionic strength: 50.000 mM; pH: 6.5; pressure: 1.000 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-EDITED 3D NOESY F1 15N/13C FILTERED | sample_new_1 | solution | sample_conditions_new_1 |
| 15N-EDITED 3D NOESY | sample_new_1 | solution | sample_conditions_new_1 |
| 13C-EDITED 3D NOESY (ALIPHATICSS) F1 15N/13C FILTERED | sample_new_1 | solution | sample_conditions_new_1 |
| 13C-EDITED 3D NOESY (ALIPHATICS) | sample_new_1 | solution | sample_conditions_new_1 |
| 13C-EDITED 3D NOESY (AROMATICS) F1 15N/13C FILTERED | sample_new_1 | solution | sample_conditions_new_1 |
| 13C-EDITED 3D NOESY (AROMATICS) | sample_new_1 | solution | sample_conditions_new_1 |
Software:
AutoDep v4.3, AutoDep - chemical shift assignment
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment
ANALYSIS v2.1, CCPN - chemical shift assignment
TALOS vany, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- BRUKER AVANCE 750 MHz
Related Database Links:
| UNP | SPF30_HUMAN |
| BMRB | 18006 |
| PDB | |
| DBJ | BAC37414 BAC41064 BAG36724 |
| EMBL | CAH93075 |
| GB | AAC64086 AAC84148 AAH11234 AAH58779 AAH97986 |
| REF | NP_001020571 NP_001030311 NP_001030414 NP_001126821 NP_001159781 |
| SP | O75940 Q3T045 Q4QQU6 Q5R591 Q8BGT7 |
| TPG | DAA14726 |
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