BMRB Entry 18017
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18017
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Title: The solution structure of RRM PubMed: 22839758
Deposition date: 2011-10-23 Original release date: 2012-08-06
Authors: Song, Zhenwei; Wu, Peiwen; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu
Citation: Song, Zhenwei; Wu, Peiwen; Ji, Peng; Zhang, Jiahai; Gong, Qingguo; Wu, Jihui; Shi, Yunyu. "Solution structure of the second RRM domain of RBM5 and its unusual binding characters for different RNA targets." Biochemistry 51, 6667-6678 (2012).
Assembly members:
entity, polymer, 95 residues, 10518.233 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: MGHHHHHHMDTIILRNIAPH
TVVDSIMTALSPYASLAVNN
IRLIKDKQTQQNRGFAFVQL
SSAMDASQLLQILQSLHPPL
KIDGKTIGVDFAKSA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 366 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 642 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RRM | 1 |
Entities:
Entity 1, RRM 95 residues - 10518.233 Da.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | MET | ASP | ||||
| 2 | THR | ILE | ILE | LEU | ARG | ASN | ILE | ALA | PRO | HIS | ||||
| 3 | THR | VAL | VAL | ASP | SER | ILE | MET | THR | ALA | LEU | ||||
| 4 | SER | PRO | TYR | ALA | SER | LEU | ALA | VAL | ASN | ASN | ||||
| 5 | ILE | ARG | LEU | ILE | LYS | ASP | LYS | GLN | THR | GLN | ||||
| 6 | GLN | ASN | ARG | GLY | PHE | ALA | PHE | VAL | GLN | LEU | ||||
| 7 | SER | SER | ALA | MET | ASP | ALA | SER | GLN | LEU | LEU | ||||
| 8 | GLN | ILE | LEU | GLN | SER | LEU | HIS | PRO | PRO | LEU | ||||
| 9 | LYS | ILE | ASP | GLY | LYS | THR | ILE | GLY | VAL | ASP | ||||
| 10 | PHE | ALA | LYS | SER | ALA |
Samples:
sample_1: RRM1.2 – 1.4 mM; sodium phosphate 40 mM; sodium chloride 50 mM; EDTA 2 mM; sodium azide 1 mM; H2O 90%; D2O 10%
sample_2: sodium phosphate 40 mM; sodium chloride 50 mM; EDTA 2 mM; sodium azide 1 mM; D2O 100%
sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
ProcheckNMR, Laskowski and MacArthur - refinement
SPARKY, Goddard - chemical shift assignment, chemical shift calculation, peak picking
NMR spectrometers:
- Bruker DMX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts