BMRB Entry 18042
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18042
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Title: Structure of EDC3:DCP2 PubMed: 22085934
Deposition date: 2011-11-07 Original release date: 2012-01-23
Authors: Truffault, Vincent
Citation: Fromm, Simon; Truffault, Vincent; Kamenz, Julia; Braun, Joerg; Hoffmann, Niklas; Izaurralde, Elisa; Sprangers, Remco. "The structural basis of Edc3- and Scd6-mediated activation of the Dcp1:Dcp2 mRNA decapping complex." EMBO J. 31, 279-290 (2011).
Assembly members:
EDC3, polymer, 96 residues, Formula weight is not available
DCP2, polymer, 48 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
EDC3: MGMSVADFYGSNVEVLLNND
SKARGVITNFDSSNSILQLR
LANDSTKSIVTKDIKDLRIL
PKNEIMPKNGTKSPSTNSTK
LKSAETYSSKNKWSMD
DCP2: GATTKEKNISVDVDADASSQ
LLSLLKSSTAPSDLATPQPS
TFPQPPVE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 436 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 678 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EDC3 | 1 |
| 2 | DCP2 | 2 |
Entities:
Entity 1, EDC3 96 residues - Formula weight is not available
| 1 | MET | GLY | MET | SER | VAL | ALA | ASP | PHE | TYR | GLY | ||||
| 2 | SER | ASN | VAL | GLU | VAL | LEU | LEU | ASN | ASN | ASP | ||||
| 3 | SER | LYS | ALA | ARG | GLY | VAL | ILE | THR | ASN | PHE | ||||
| 4 | ASP | SER | SER | ASN | SER | ILE | LEU | GLN | LEU | ARG | ||||
| 5 | LEU | ALA | ASN | ASP | SER | THR | LYS | SER | ILE | VAL | ||||
| 6 | THR | LYS | ASP | ILE | LYS | ASP | LEU | ARG | ILE | LEU | ||||
| 7 | PRO | LYS | ASN | GLU | ILE | MET | PRO | LYS | ASN | GLY | ||||
| 8 | THR | LYS | SER | PRO | SER | THR | ASN | SER | THR | LYS | ||||
| 9 | LEU | LYS | SER | ALA | GLU | THR | TYR | SER | SER | LYS | ||||
| 10 | ASN | LYS | TRP | SER | MET | ASP |
Entity 2, DCP2 48 residues - Formula weight is not available
| 1 | GLY | ALA | THR | THR | LYS | GLU | LYS | ASN | ILE | SER | ||||
| 2 | VAL | ASP | VAL | ASP | ALA | ASP | ALA | SER | SER | GLN | ||||
| 3 | LEU | LEU | SER | LEU | LEU | LYS | SER | SER | THR | ALA | ||||
| 4 | PRO | SER | ASP | LEU | ALA | THR | PRO | GLN | PRO | SER | ||||
| 5 | THR | PHE | PRO | GLN | PRO | PRO | VAL | GLU |
Samples:
sample_1: EDC3, [U-100% 15N], 0.7 mM; DCP2, [U-100% 15N], 0.7 mM; NaPO4 50 mM; imidazole 10 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 18041 |
| PDB | |
| EMBL | CAA22671 CAB11648 |
| REF | NP_595858 NP_593780 |
| SP | O94752 O13828 |
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