BMRB Entry 18125
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18125
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Title: Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms PubMed: 22392339
Deposition date: 2011-12-08 Original release date: 2012-03-09
Authors: Tossavainen, Helena; Helppolainen, Satu; Maatta, Juha; Pihlajamaa, Tero; Hytonen, Vesa; Kulomaa, Markku; Permi, Perttu
Citation: Tossavainen, Helena; Helppolainen, Satu; Maatta, Juha; Pihlajamaa, Tero; Hytonen, Vesa; Kulomaa, Markku; Permi, Perttu. "Resonance assignments of the 56kDa chimeric avidin in the biotin-bound and free forms." Biomol. NMR Assignments 7, 35-38 (2013).
Assembly members:
avidin, polymer, 129 residues, Formula weight is not available
BTN, non-polymer, 244.311 Da.
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
avidin: QTVARKCSLTGKWTNDLGSN
MTIGAVNSRGEFTGTYITAV
ADNPGNITLSPLLGIQHKRA
SQPTFGFTVNWKFSESTTVF
TGQCFIDRNGKEVLKTMWLL
RSSVNDIGDDWKATRVGYNI
FTRLRTQKE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 418 |
| 15N chemical shifts | 120 |
| 1H chemical shifts | 851 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | avidin | 1 |
| 2 | BTN | 1 |
Entities:
Entity 1, avidin 129 residues - Formula weight is not available
| 1 | GLN | THR | VAL | ALA | ARG | LYS | CYS | SER | LEU | THR | ||||
| 2 | GLY | LYS | TRP | THR | ASN | ASP | LEU | GLY | SER | ASN | ||||
| 3 | MET | THR | ILE | GLY | ALA | VAL | ASN | SER | ARG | GLY | ||||
| 4 | GLU | PHE | THR | GLY | THR | TYR | ILE | THR | ALA | VAL | ||||
| 5 | ALA | ASP | ASN | PRO | GLY | ASN | ILE | THR | LEU | SER | ||||
| 6 | PRO | LEU | LEU | GLY | ILE | GLN | HIS | LYS | ARG | ALA | ||||
| 7 | SER | GLN | PRO | THR | PHE | GLY | PHE | THR | VAL | ASN | ||||
| 8 | TRP | LYS | PHE | SER | GLU | SER | THR | THR | VAL | PHE | ||||
| 9 | THR | GLY | GLN | CYS | PHE | ILE | ASP | ARG | ASN | GLY | ||||
| 10 | LYS | GLU | VAL | LEU | LYS | THR | MET | TRP | LEU | LEU | ||||
| 11 | ARG | SER | SER | VAL | ASN | ASP | ILE | GLY | ASP | ASP | ||||
| 12 | TRP | LYS | ALA | THR | ARG | VAL | GLY | TYR | ASN | ILE | ||||
| 13 | PHE | THR | ARG | LEU | ARG | THR | GLN | LYS | GLU |
Samples:
sample_1: avidin, [U-13C; U-15N], 0.7 – 1.2 mM; d-biotin0.7 – 1.2 mM; H2O 93%; D2O 7%
sample_2: avidin, [U-13C; U-15N], 0.7 – 1.2 mM; H2O 93%; D2O 7%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 333 K
sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 343 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| (HB)CB(CGCD)HD | sample_1 | isotropic | sample_conditions_1 |
| HCCmHm | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection, processing
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian Unity INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts