BMRB Entry 18132
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18132
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Title: THERMOSTABLE PROTEIN FROM HYPERTHERMOPHILIC VIRUS SSV-RH PubMed: 22409376
Deposition date: 2011-12-09 Original release date: 2012-03-23
Authors: Schlenker, C.; Goel, Anupam; Tripet, B.; Menon, S.; Lawrence, C.; Copie, V.
Citation: Schlenker, Casey; Goel, Anupam; Tripet, Brian; Menon, Smita; Willi, Taylor; Dlakic, Mensur; Young, Mark; Lawrence, C. Martin; Copie, Valerie. "Structural studies of E73 from a hyperthermophilic archaeal virus identify the "RH3" domain, an elaborated ribbon-helix-helix motif involved in DNA recognition." Biochemistry 51, 2899-2910 (2012).
Assembly members:
ORF_E73, polymer, 73 residues, 8627.1995 Da.
Natural source: Common Name: Sulfolobus virus Ragged Hills Taxonomy ID: 256994 Superkingdom: Viruses Kingdom: not available Genus/species: Fusellovirus Sulfolobus virus Ragged Hills
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ORF_E73: MVESKKIAKKKTTLAFDEDV
YHTLKLVSVYLNRDMTEIIE
EAVVMWLIQNKEKLPNELKP
KIDEISKRFFPAK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 171 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 335 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ORF E73, chain 1 | 1 |
| 2 | ORF E73, chain 2 | 1 |
Entities:
Entity 1, ORF E73, chain 1 73 residues - 8627.1995 Da.
| 1 | MET | VAL | GLU | SER | LYS | LYS | ILE | ALA | LYS | LYS | ||||
| 2 | LYS | THR | THR | LEU | ALA | PHE | ASP | GLU | ASP | VAL | ||||
| 3 | TYR | HIS | THR | LEU | LYS | LEU | VAL | SER | VAL | TYR | ||||
| 4 | LEU | ASN | ARG | ASP | MET | THR | GLU | ILE | ILE | GLU | ||||
| 5 | GLU | ALA | VAL | VAL | MET | TRP | LEU | ILE | GLN | ASN | ||||
| 6 | LYS | GLU | LYS | LEU | PRO | ASN | GLU | LEU | LYS | PRO | ||||
| 7 | LYS | ILE | ASP | GLU | ILE | SER | LYS | ARG | PHE | PHE | ||||
| 8 | PRO | ALA | LYS |
Samples:
sample_1: PMSF, [U-13C; U-15N], 1 mM; SODIUM AZIDE 0.01%; POTASSIUM PHOSPHATE 50 mM; EDTA 1 mM
sample_conditions_1: ionic strength: 0.050 mM; pH: 5.000; pressure: 1.000 atm; temperature: 312.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | solution | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | solution | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | solution | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | solution | sample_conditions_1 |
| 3D HNCA | sample_1 | solution | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | solution | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | solution | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | solution | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | solution | sample_conditions_1 |
Software:
ARIA vany, Linge, O, . - chemical shift assignment
AutoDep v4.3, PDBe - collection
CNS vany, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - chemical shift assignment
NMR spectrometers:
- Bruker DMX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts