BMRB

Biological Magnetic Resonance Data Bank


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BMRB Entry 18211

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18211
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Title: 1H, 15N and 13C resonance assignments for the LOTUS2 and LOTUS3 RNA binding domains of mouse TDRD7   PubMed: 22481467

Deposition date: 2012-01-23 Original release date: 2012-03-20

Authors: Cui, Gaofeng; Botuyan, Maria Victoria; Mer, Georges

Citation: Cui, Gaofeng; Botuyan, Maria Victoria; Mer, Georges. "(1)H, (15)N and (13)C resonance assignments for the three LOTUS RNA binding domains of Tudor domain-containing protein TDRD7."  Biomol. NMR Assignments 7, 79-83 (2013).

Assembly members:
TDRD7, polymer, 180 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TDRD7: GHMDEVQNRIKEILDKHNNG IWISKLPHFYKEFYKEDLNQ GVLQQFEHWPHICTVEKPCG GGQDSLLYPARREQPLKSDQ DPEKELPPPPPAPKQEVPSQ GSPAVMPDVKEKVAELLGKY SSGLWASALPKAFEDMYKVK FPEDALKNLASLSDVCTINY ISGNTQKAILYAKLPLPTDK

Data sets:
Data typeCount
13C chemical shifts834
15N chemical shifts179
1H chemical shifts1316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Lotus domains 2 and 31

Entities:

Entity 1, Lotus domains 2 and 3 180 residues - Formula weight is not available

1   GLYHISMETASPGLUVALGLNASNARGILE
2   LYSGLUILELEUASPLYSHISASNASNGLY
3   ILETRPILESERLYSLEUPROHISPHETYR
4   LYSGLUPHETYRLYSGLUASPLEUASNGLN
5   GLYVALLEUGLNGLNPHEGLUHISTRPPRO
6   HISILECYSTHRVALGLULYSPROCYSGLY
7   GLYGLYGLNASPSERLEULEUTYRPROALA
8   ARGARGGLUGLNPROLEULYSSERASPGLN
9   ASPPROGLULYSGLULEUPROPROPROPRO
10   PROALAPROLYSGLNGLUVALPROSERGLN
11   GLYSERPROALAVALMETPROASPVALLYS
12   GLULYSVALALAGLULEULEUGLYLYSTYR
13   SERSERGLYLEUTRPALASERALALEUPRO
14   LYSALAPHEGLUASPMETTYRLYSVALLYS
15   PHEPROGLUASPALALEULYSASNLEUALA
16   SERLEUSERASPVALCYSTHRILEASNTYR
17   ILESERGLYASNTHRGLNLYSALAILELEU
18   TYRALALYSLEUPROLEUPROTHRASPLYS

Samples:

sample_1: TDRD7 (LOTUS1-LOTUS2), [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.26 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
GB AAH29689
REF NP_001277404 NP_666254 XP_006537580 XP_006537581
SP Q8K1H1

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