BMRB Entry 18225

Name: AR55 solubilised in DPC micelles
Published: 2013-01-14

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PDB ID: 2lou
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18225
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: AR55 solubilised in DPC micelles PubMed: 23438363

Deposition date: 2012-01-27 Original release date: 2013-01-14

Authors: Langelaan, David; Rainey, Jan

Citation: Langelaan, David; Reddy, Tyler; Banks, Aaron; Dellaire, Graham; Dupre, Denis; Rainey, Jan. "Structural features of the apelin receptor N-terminal tail and first transmembrane segment implicated in ligand binding and receptor trafficking" Biochim. Biophys. Acta 1828, 1471-1483 (2013).

Assembly members:
AR55, polymer, 64 residues, 7298.1072 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
AR55: MEEGGDFDNYYGADNQSECE YTDWKSSGALIPAIYMLVFL LGTTGNGLVLWTVFRKKGHH HHHH

Data sets:

Data type	Count
13C chemical shifts	281
15N chemical shifts	70
1H chemical shifts	404

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AR55	1

Entities:

Entity 1, AR55 64 residues - 7298.1072 Da.

1

MET

GLU

GLY

ASP

PHE

ASP

ASN

TYR

2

TYR

GLY

ALA

ASP

ASN

GLN

SER

GLU

CYS

GLU

3

TYR

THR

ASP

TRP

LYS

SER

GLY

ALA

LEU

4

ILE

PRO

ALA

ILE

TYR

MET

LEU

VAL

PHE

LEU

5

LEU

GLY

THR

GLY

ASN

GLY

LEU

VAL

LEU

6

TRP

THR

VAL

PHE

ARG

LYS

GLY

HIS

7

HIS

Samples:

sample_1: AR55, [U-99% 13C; U-99% 15N], 1 mM; DPC, [U-99% 2H], 150 mM; sodium acetate 20 mM; sodium azide 1 mM; DSS 1 mM; DTT, [U-99% 2H], 10 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 4; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC/HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
13Cfilt_NOESY (H[{N\|C}]_H.NOESY)	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC/HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
13CHSQC_arom*800 (H[C[caro]])	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

ANALYSIS v2.1, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 800 MHz
Bruker Avance III 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts