BMRB Entry 18242
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18242
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Title: Backbone and sidechain 1H, 15N and 13C assignments of NLRP10 (PYNOD) pyrin domain PubMed: 22618865
Deposition date: 2012-02-07 Original release date: 2012-06-05
Authors: Chang, Chung-I; Chang, Chi-Fon; Su, Ming-Yuan
Citation: Su, Ming-Yuan; Chang, Chung-I; Chang, Chi-Fon. "1H, 13C and 15N resonance assignments of the pyrin domain from human PYNOD." Biomol. NMR Assignments 7, 141-143 (2013).
Assembly members:
PYNOD_PYD, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PYNOD_PYD: GAMAMAKARKPREALLWALS
DLEENDFKKLKFYLRDMTLS
EGQPPLARGELEGLIPVDLA
ELLISKYGEKEAVKVVLKGL
KVMNLLELVDQLSHICLHDY
RE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 428 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 719 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PYNOD PYD | 1 |
Entities:
Entity 1, PYNOD PYD 102 residues - Formula weight is not available
| 1 | GLY | ALA | MET | ALA | MET | ALA | LYS | ALA | ARG | LYS | ||||
| 2 | PRO | ARG | GLU | ALA | LEU | LEU | TRP | ALA | LEU | SER | ||||
| 3 | ASP | LEU | GLU | GLU | ASN | ASP | PHE | LYS | LYS | LEU | ||||
| 4 | LYS | PHE | TYR | LEU | ARG | ASP | MET | THR | LEU | SER | ||||
| 5 | GLU | GLY | GLN | PRO | PRO | LEU | ALA | ARG | GLY | GLU | ||||
| 6 | LEU | GLU | GLY | LEU | ILE | PRO | VAL | ASP | LEU | ALA | ||||
| 7 | GLU | LEU | LEU | ILE | SER | LYS | TYR | GLY | GLU | LYS | ||||
| 8 | GLU | ALA | VAL | LYS | VAL | VAL | LEU | LYS | GLY | LEU | ||||
| 9 | LYS | VAL | MET | ASN | LEU | LEU | GLU | LEU | VAL | ASP | ||||
| 10 | GLN | LEU | SER | HIS | ILE | CYS | LEU | HIS | ASP | TYR | ||||
| 11 | ARG | GLU |
Samples:
sample_1: PYNOD PYD, [U-13C; U-15N], 0.2 mM
sample_conditions_1: ionic strength: 140 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| GB | AAI04958 AAO18161 AAS67384 AIC53619 EAW68640 |
| REF | NP_789791 XP_003254944 XP_003313013 XP_003818292 XP_004050717 |
| SP | Q86W26 |
| TPG | DAA01243 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts