BMRB Entry 18338
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18338
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Title: C-terminal propeptide (PPc) region of vibrio extracellular metalloprotease PubMed: 23228665
Deposition date: 2012-03-20 Original release date: 2013-01-03
Authors: Yun, Ji-Hye
Citation: Yun, Ji-Hye; Kim, Heeyoun; Park, Jung Eun; Lee, Jung Sup; Lee, Weontae. "Solution structure and dynamics of C-terminal regulatory domain of Vibrio vulnificus extracellular metalloprotease." Biochem. Biophys. Res. Commun. 430, 541-546 (2013).
Assembly members:
C-terminal_propeptide_(PPc)_region_of_vibrio_extracellular_metalloprotease, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: vibrio vulnificus Taxonomy ID: 672 Superkingdom: Bacteria Kingdom: not available Genus/species: vibrio vulnificus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
C-terminal_propeptide_(PPc)_region_of_vibrio_extracellular_metalloprotease: GNVLKNNTPVSNLTGNKGSE
VFYTFTVDRNATAVVSISGG
SGDADLYLKAGSKPTTSSWD
CRPYRYGNNESCSVSAAPGT
TYHVMIKGYSNYSGVTLKLQ
Y
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 368 |
| 15N chemical shifts | 102 |
| 1H chemical shifts | 631 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PPc | 1 |
Entities:
Entity 1, PPc 101 residues - Formula weight is not available
| 1 | GLY | ASN | VAL | LEU | LYS | ASN | ASN | THR | PRO | VAL | ||||
| 2 | SER | ASN | LEU | THR | GLY | ASN | LYS | GLY | SER | GLU | ||||
| 3 | VAL | PHE | TYR | THR | PHE | THR | VAL | ASP | ARG | ASN | ||||
| 4 | ALA | THR | ALA | VAL | VAL | SER | ILE | SER | GLY | GLY | ||||
| 5 | SER | GLY | ASP | ALA | ASP | LEU | TYR | LEU | LYS | ALA | ||||
| 6 | GLY | SER | LYS | PRO | THR | THR | SER | SER | TRP | ASP | ||||
| 7 | CYS | ARG | PRO | TYR | ARG | TYR | GLY | ASN | ASN | GLU | ||||
| 8 | SER | CYS | SER | VAL | SER | ALA | ALA | PRO | GLY | THR | ||||
| 9 | THR | TYR | HIS | VAL | MET | ILE | LYS | GLY | TYR | SER | ||||
| 10 | ASN | TYR | SER | GLY | VAL | THR | LEU | LYS | LEU | GLN | ||||
| 11 | TYR |
Samples:
PPc_domain_of_vEP: PPc protein, [U-100% 15N], 0.6 mM; HEPES (pH 7.0) 20 mM; Sodium chloride 100 mM; DTT 10 mM; NaN3 0.01%; H2O 90%; D2O 10%
PPc_domain_of_vEP_2: PPc protein, [U-100% 13C; U-100% 15N], 0.6 mM; HEPES (pH 7.0) 20 mM; Sodium chloride 100 mM; DTT 10 mM; NaN3 0.01%; H2O 90%; D2O 10%
0.6mM_PPc_protein: ionic strength: 20 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | PPc_domain_of_vEP | isotropic | 0.6mM_PPc_protein |
| 3D 1H-15N NOESY | PPc_domain_of_vEP | isotropic | 0.6mM_PPc_protein |
| 3D 1H-13C NOESY | PPc_domain_of_vEP_2 | isotropic | 0.6mM_PPc_protein |
| 3D HNCACB | PPc_domain_of_vEP_2 | isotropic | 0.6mM_PPc_protein |
| 3D HBHA(CO)NH | PPc_domain_of_vEP_2 | isotropic | 0.6mM_PPc_protein |
| 3D HNCA | PPc_domain_of_vEP_2 | isotropic | 0.6mM_PPc_protein |
| 3D HNCO | PPc_domain_of_vEP_2 | isotropic | 0.6mM_PPc_protein |
| 3D HBHA(CO)NH | PPc_domain_of_vEP_2 | isotropic | 0.6mM_PPc_protein |
| 3D HCCH-TOCSY | PPc_domain_of_vEP_2 | isotropic | 0.6mM_PPc_protein |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAC22940 BAC97491 BAI66359 BAI66360 BAI66361 |
| EMBL | CAM34546 |
| GB | AAC44789 AAC45343 AAC72410 AAO07886 ABI95803 |
| REF | WP_011081880 WP_015728348 WP_026050529 WP_026130953 WP_038940484 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts